Abstract
Ferric citrate induces the ferric citrate transport system in Escherichia coli without being taken up into cells. The cytoplasmic transmembrane protein FecR, required for the response to ferric citrate, was found to be cleaved by a cellular protease. FecR protein produced by fecR mutants impaired or constitutive in fecA transcription was protease resistant, indicating that conformational changes affect proper functioning of FecR.
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- Giacomini A., Corich V., Ollero F. J., Squartini A., Nuti M. P. Experimental conditions may affect reproducibility of the beta-galactosidase assay. FEMS Microbiol Lett. 1992 Dec 15;100(1-3):87–90. doi: 10.1111/j.1574-6968.1992.tb14024.x. [DOI] [PubMed] [Google Scholar]
- Lonetto M. A., Brown K. L., Rudd K. E., Buttner M. J. Analysis of the Streptomyces coelicolor sigE gene reveals the existence of a subfamily of eubacterial RNA polymerase sigma factors involved in the regulation of extracytoplasmic functions. Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7573–7577. doi: 10.1073/pnas.91.16.7573. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lugtenberg B., Meijers J., Peters R., van der Hoek P., van Alphen L. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands. FEBS Lett. 1975 Oct 15;58(1):254–258. doi: 10.1016/0014-5793(75)80272-9. [DOI] [PubMed] [Google Scholar]
- Matsudaira P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem. 1987 Jul 25;262(21):10035–10038. [PubMed] [Google Scholar]
- Ochs M., Veitinger S., Kim I., Welz D., Angerer A., Braun V. Regulation of citrate-dependent iron transport of Escherichia coli: fecR is required for transcription activation by FecI. Mol Microbiol. 1995 Jan;15(1):119–132. doi: 10.1111/j.1365-2958.1995.tb02226.x. [DOI] [PubMed] [Google Scholar]
- Stappert J., Wirsching J., Kemler R. A PCR method for introducing mutations into cloned DNA by joining an internal primer to a tagged flanking primer. Nucleic Acids Res. 1992 Feb 11;20(3):624–624. doi: 10.1093/nar/20.3.624. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Studier F. W., Moffatt B. A. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol. 1986 May 5;189(1):113–130. doi: 10.1016/0022-2836(86)90385-2. [DOI] [PubMed] [Google Scholar]
- Tabor S., Richardson C. C. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074–1078. doi: 10.1073/pnas.82.4.1074. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Hove B., Staudenmaier H., Braun V. Novel two-component transmembrane transcription control: regulation of iron dicitrate transport in Escherichia coli K-12. J Bacteriol. 1990 Dec;172(12):6749–6758. doi: 10.1128/jb.172.12.6749-6758.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zimmermann L., Hantke K., Braun V. Exogenous induction of the iron dicitrate transport system of Escherichia coli K-12. J Bacteriol. 1984 Jul;159(1):271–277. doi: 10.1128/jb.159.1.271-277.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]