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. 1995 Jul;177(13):3879–3884. doi: 10.1128/jb.177.13.3879-3884.1995

Functional characterization and transcriptional analysis of a gene cluster governing early and late steps in daunorubicin biosynthesis in Streptomyces peucetius.

K Madduri 1, C R Hutchinson 1
PMCID: PMC177111  PMID: 7601857

Abstract

Sequence analysis of the Streptomyces peucetius daunorubicin biosynthetic gene cluster revealed a partial (dnrQ) and two complete (dnrD and dnrP) open reading frames flanking dnrK. Bioconversion experiments showed that DnrD converts aklanonic acid methylester to aklaviketone and that DnrC is a methyltransferase that converts aklanonic acid to aklanonic acid methylester. The deduced dnrP gene product, homologous to known esterases, may catalyze the conversion of 10-carbomethoxy-13-deoxycarminomycin to its 10-carboxy derivative. The dnrKPQS genes may be transcribed as a polycistronic mRNA.

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Selected References

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