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. 1995 Jul;177(14):4077–4083. doi: 10.1128/jb.177.14.4077-4083.1995

Incomplete entry of bacteriophage T7 DNA into F plasmid-containing Escherichia coli.

L R García 1, I J Molineux 1
PMCID: PMC177139  PMID: 7608082

Abstract

The penetration of bacteriophage T7 DNA into F plasmid-containing Escherichia coli cells was determined by measuring Dam methylation of the entering genome. T7 strains that cannot productively infect F-containing cells fail to completely translocate their DNA into the cell before the infection aborts. The entry of the first 44% of the genome occurs normally in an F-containing cell, but the entry of the remainder is aberrant. Bypassing the normal mode of entry of the T7 genome by transfecting naked DNA into competent cells fails to suppress F exclusion of phage development. However, overexpression of various nontoxic T7 1.2 alleles from a high-copy-number plasmid or expression of T3 1.2 from a T7 genome allows phage growth in the presence of F.

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Selected References

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