Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Journal of Bacteriology logoLink to Journal of Bacteriology
. 1995 Jul;177(14):4137–4139. doi: 10.1128/jb.177.14.4137-4139.1995

Oligoribonuclease is distinct from the other known exoribonucleases of Escherichia coli.

D Yu 1, M P Deutscher 1
PMCID: PMC177148  PMID: 7608090

Abstract

Oligoribonuclease, an exoribonuclease specific for small oligoribonucleotides, was initially characterized 20 years ago (S. K. Niyogi and A. K. Datta, J. Biol. Chem. 250:7307-7312, 1975) and shown to be different from RNase II and polynucleotide phosphorylase. Here we demonstrate, using mutant strains and purified enzymes, that oligoribonuclease is not a manifestation of RNases D, BN, T, PH, and R, exoribonucleases discovered subsequently. Thus, oligoribonuclease is the eighth distinct exoribonuclease discovered in Escherichia coli. We also show that oligoribonuclease copurifies with polynucleotide phosphorylase.

Full Text

The Full Text of this article is available as a PDF (193.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. Carpousis A. J., Van Houwe G., Ehretsmann C., Krisch H. M. Copurification of E. coli RNAase E and PNPase: evidence for a specific association between two enzymes important in RNA processing and degradation. Cell. 1994 Mar 11;76(5):889–900. doi: 10.1016/0092-8674(94)90363-8. [DOI] [PubMed] [Google Scholar]
  3. Cudny H., Deutscher M. P. Apparent involvement of ribonuclease D in the 3' processing of tRNA precursors. Proc Natl Acad Sci U S A. 1980 Feb;77(2):837–841. doi: 10.1073/pnas.77.2.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Datta A. K., Niyogi K. A novel oligoribonuclease of Escherichia coli. II. Mechanism of action. J Biol Chem. 1975 Sep 25;250(18):7313–7319. [PubMed] [Google Scholar]
  5. Deutscher M. P., Marlor C. W. Purification and characterization of Escherichia coli RNase T. J Biol Chem. 1985 Jun 10;260(11):7067–7071. [PubMed] [Google Scholar]
  6. Deutscher M. P., Marlor C. W., Zaniewski R. Ribonuclease T: new exoribonuclease possibly involved in end-turnover of tRNA. Proc Natl Acad Sci U S A. 1984 Jul;81(14):4290–4293. doi: 10.1073/pnas.81.14.4290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Deutscher M. P. Promiscuous exoribonucleases of Escherichia coli. J Bacteriol. 1993 Aug;175(15):4577–4583. doi: 10.1128/jb.175.15.4577-4583.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Deutscher M. P. Ribonuclease multiplicity, diversity, and complexity. J Biol Chem. 1993 Jun 25;268(18):13011–13014. [PubMed] [Google Scholar]
  9. Jensen K. F., Andersen J. T., Poulsen P. Overexpression and rapid purification of the orfE/rph gene product, RNase PH of Escherichia coli. J Biol Chem. 1992 Aug 25;267(24):17147–17152. [PubMed] [Google Scholar]
  10. Kelly K. O., Deutscher M. P. Characterization of Escherichia coli RNase PH. J Biol Chem. 1992 Aug 25;267(24):17153–17158. [PubMed] [Google Scholar]
  11. Kelly K. O., Reuven N. B., Li Z., Deutscher M. P. RNase PH is essential for tRNA processing and viability in RNase-deficient Escherichia coli cells. J Biol Chem. 1992 Aug 15;267(23):16015–16018. [PubMed] [Google Scholar]
  12. Li Z., Deutscher M. P. The role of individual exoribonucleases in processing at the 3' end of Escherichia coli tRNA precursors. J Biol Chem. 1994 Feb 25;269(8):6064–6071. [PubMed] [Google Scholar]
  13. Niyogi S. K., Datta A. K. A novel oligoribonuclease of Escherichia coli. I. Isolation and properties. J Biol Chem. 1975 Sep 25;250(18):7307–7312. [PubMed] [Google Scholar]
  14. Nossal N. G., Singer M. F. The processive degradation of individual polyribonucleotide chains. I. Escherichia coli ribonuclease II. J Biol Chem. 1968 Mar 10;243(5):913–922. [PubMed] [Google Scholar]
  15. Reuven N. B., Deutscher M. P. Multiple exoribonucleases are required for the 3' processing of Escherichia coli tRNA precursors in vivo. FASEB J. 1993 Jan;7(1):143–148. doi: 10.1096/fasebj.7.1.8422961. [DOI] [PubMed] [Google Scholar]
  16. Zhang J. R., Deutscher M. P. Escherichia coli RNase D: sequencing of the rnd structural gene and purification of the overexpressed protein. Nucleic Acids Res. 1988 Jul 25;16(14A):6265–6278. doi: 10.1093/nar/16.14.6265. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES