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. 1995 Jul;177(14):4166–4170. doi: 10.1128/jb.177.14.4166-4170.1995

Ser-127-to-Leu substitution in the DNA gyrase B subunit of Streptococcus pneumoniae is implicated in novobiocin resistance.

R Muñoz 1, M Bustamante 1, A G de la Campa 1
PMCID: PMC177155  PMID: 7608096

Abstract

We report the cloning of the gyrB gene from Streptococcus pneumoniae 533 that carries the nov-1 allele. The gyrB gene codes for a protein homologous to the gyrase B subunit of archaebacteria and eubacteria. The same amino acid substitution (Ser-127 to Leu) confers novobiocin resistance on four isolates of S. pneumoniae. This amino acid position is equivalent to Val-120 of Escherichia coli GyrB, a residue that lies inside the ATP-binding domain as revealed by the crystal structure of the protein.

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Selected References

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