Abstract
XerC and XerD are site-specific recombinases of the lambda integrase family which resolve multimeric replicons to monomers by acting at specific sites such as cer, ckr, nmr, parB, and psi, which are found in plasmids, or at the dif site found in the Escherichia coli chromosome. By using Southern hybridizations to cloned E. coli xerC and xerD genes and a cer-nmr plasmid-based resolution assay, the presence of these genes in several species of Enterobacteriaceae is shown.
Full Text
The Full Text of this article is available as a PDF (486.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blakely G., Colloms S., May G., Burke M., Sherratt D. Escherichia coli XerC recombinase is required for chromosomal segregation at cell division. New Biol. 1991 Aug;3(8):789–798. [PubMed] [Google Scholar]
- Blakely G., May G., McCulloch R., Arciszewska L. K., Burke M., Lovett S. T., Sherratt D. J. Two related recombinases are required for site-specific recombination at dif and cer in E. coli K12. Cell. 1993 Oct 22;75(2):351–361. doi: 10.1016/0092-8674(93)80076-q. [DOI] [PubMed] [Google Scholar]
- Cannon P. M., Strike P. Complete nucleotide sequence and gene organization of plasmid NTP16. Plasmid. 1992 May;27(3):220–230. doi: 10.1016/0147-619x(92)90024-5. [DOI] [PubMed] [Google Scholar]
- Colloms S. D., Sykora P., Szatmari G., Sherratt D. J. Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases. J Bacteriol. 1990 Dec;172(12):6973–6980. doi: 10.1128/jb.172.12.6973-6980.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cornet F., Mortier I., Patte J., Louarn J. M. Plasmid pSC101 harbors a recombination site, psi, which is able to resolve plasmid multimers and to substitute for the analogous chromosomal Escherichia coli site dif. J Bacteriol. 1994 Jun;176(11):3188–3195. doi: 10.1128/jb.176.11.3188-3195.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guzzo A., DuBow M. S. Construction of stable, single-copy luciferase gene fusions in Escherichia coli. Arch Microbiol. 1991;156(6):444–448. doi: 10.1007/BF00245390. [DOI] [PubMed] [Google Scholar]
- Hakkaart M. J., van den Elzen P. J., Veltkamp E., Nijkamp H. J. Maintenance of multicopy plasmid Clo DF13 in E. coli cells: evidence for site-specific recombination at parB. Cell. 1984 Jan;36(1):203–209. doi: 10.1016/0092-8674(84)90090-4. [DOI] [PubMed] [Google Scholar]
- Kuempel P. L., Henson J. M., Dircks L., Tecklenburg M., Lim D. F. dif, a recA-independent recombination site in the terminus region of the chromosome of Escherichia coli. New Biol. 1991 Aug;3(8):799–811. [PubMed] [Google Scholar]
- McCulloch R., Coggins L. W., Colloms S. D., Sherratt D. J. Xer-mediated site-specific recombination at cer generates Holliday junctions in vivo. EMBO J. 1994 Apr 15;13(8):1844–1855. doi: 10.1002/j.1460-2075.1994.tb06453.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stirling C. J., Colloms S. D., Collins J. F., Szatmari G., Sherratt D. J. xerB, an Escherichia coli gene required for plasmid ColE1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine aminopeptidase. EMBO J. 1989 May;8(5):1623–1627. doi: 10.1002/j.1460-2075.1989.tb03547.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stirling C. J., Stewart G., Sherratt D. J. Multicopy plasmid stability in Escherichia coli requires host-encoded functions that lead to plasmid site-specific recombination. Mol Gen Genet. 1988 Sep;214(1):80–84. doi: 10.1007/BF00340183. [DOI] [PubMed] [Google Scholar]
- Summers D. K., Sherratt D. J. Multimerization of high copy number plasmids causes instability: CoIE1 encodes a determinant essential for plasmid monomerization and stability. Cell. 1984 Apr;36(4):1097–1103. doi: 10.1016/0092-8674(84)90060-6. [DOI] [PubMed] [Google Scholar]
- Summers D. K., Sherratt D. J. Resolution of ColE1 dimers requires a DNA sequence implicated in the three-dimensional organization of the cer site. EMBO J. 1988 Mar;7(3):851–858. doi: 10.1002/j.1460-2075.1988.tb02884.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Summers D., Yaish S., Archer J., Sherratt D. Multimer resolution systems of ColE1 and ColK: localisation of the crossover site. Mol Gen Genet. 1985;201(2):334–338. doi: 10.1007/BF00425680. [DOI] [PubMed] [Google Scholar]