Abstract
AbrB is a transcriptional regulator of many Bacillus subtilis genes. A number of AbrB-binding sites have previously been delimited by DNase I footprinting studies, but the heterogeneity of the protected sequences and sizes has not led to a determination of a possible consensus motif for recognition. We have examined the affinity of AbrB for binding to six known target regions when the regions were placed in DNA fragments of various sizes. The sites are shown to vary dramatically in AbrB-binding affinity when they are present in smaller fragments, but the differences are smaller when the affinities of larger fragments are compared. Additional observations that indicate that AbrB binding may be a multistep cooperative process are reported.
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- Brosius J. Superpolylinkers in cloning and expression vectors. DNA. 1989 Dec;8(10):759–777. doi: 10.1089/dna.1989.8.759. [DOI] [PubMed] [Google Scholar]
- Fisher S. H., Strauch M. A., Atkinson M. R., Wray L. V., Jr Modulation of Bacillus subtilis catabolite repression by transition state regulatory protein AbrB. J Bacteriol. 1994 Apr;176(7):1903–1912. doi: 10.1128/jb.176.7.1903-1912.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fürbass R., Gocht M., Zuber P., Marahiel M. A. Interaction of AbrB, a transcriptional regulator from Bacillus subtilis with the promoters of the transition state-activated genes tycA and spoVG. Mol Gen Genet. 1991 Mar;225(3):347–354. doi: 10.1007/BF00261673. [DOI] [PubMed] [Google Scholar]
- Gaur N. K., Cabane K., Smith I. Structure and expression of the Bacillus subtilis sin operon. J Bacteriol. 1988 Mar;170(3):1046–1053. doi: 10.1128/jb.170.3.1046-1053.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Henner D. J., Ferrari E., Perego M., Hoch J. A. Location of the targets of the hpr-97, sacU32(Hy), and sacQ36(Hy) mutations in upstream regions of the subtilisin promoter. J Bacteriol. 1988 Jan;170(1):296–300. doi: 10.1128/jb.170.1.296-300.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Perego M., Hoch J. A. Isolation and sequence of the spo0E gene: its role in initiation of sporulation in Bacillus subtilis. Mol Microbiol. 1987 Jul;1(1):125–132. doi: 10.1111/j.1365-2958.1987.tb00536.x. [DOI] [PubMed] [Google Scholar]
- Perego M., Spiegelman G. B., Hoch J. A. Structure of the gene for the transition state regulator, abrB: regulator synthesis is controlled by the spo0A sporulation gene in Bacillus subtilis. Mol Microbiol. 1988 Nov;2(6):689–699. doi: 10.1111/j.1365-2958.1988.tb00079.x. [DOI] [PubMed] [Google Scholar]
- Slack F. J., Mueller J. P., Sonenshein A. L. Mutations that relieve nutritional repression of the Bacillus subtilis dipeptide permease operon. J Bacteriol. 1993 Aug;175(15):4605–4614. doi: 10.1128/jb.175.15.4605-4614.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Slack F. J., Mueller J. P., Strauch M. A., Mathiopoulos C., Sonenshein A. L. Transcriptional regulation of a Bacillus subtilis dipeptide transport operon. Mol Microbiol. 1991 Aug;5(8):1915–1925. doi: 10.1111/j.1365-2958.1991.tb00815.x. [DOI] [PubMed] [Google Scholar]
- Strauch M. A., Ayazifar M. Bent DNA is found in some, but not all, regions recognized by the Bacillus subtilis AbrB protein. Mol Gen Genet. 1995 Mar 20;246(6):756–760. doi: 10.1007/BF00290723. [DOI] [PubMed] [Google Scholar]
- Strauch M. A., Hoch J. A. Transition-state regulators: sentinels of Bacillus subtilis post-exponential gene expression. Mol Microbiol. 1993 Feb;7(3):337–342. doi: 10.1111/j.1365-2958.1993.tb01125.x. [DOI] [PubMed] [Google Scholar]
- Strauch M. A., Perego M., Burbulys D., Hoch J. A. The transition state transcription regulator AbrB of Bacillus subtilis is autoregulated during vegetative growth. Mol Microbiol. 1989 Sep;3(9):1203–1209. doi: 10.1111/j.1365-2958.1989.tb00270.x. [DOI] [PubMed] [Google Scholar]
- Strauch M. A. Regulation of Bacillus subtilis gene expression during the transition from exponential growth to stationary phase. Prog Nucleic Acid Res Mol Biol. 1993;46:121–153. doi: 10.1016/s0079-6603(08)61020-x. [DOI] [PubMed] [Google Scholar]
- Strauch M. A., Spiegelman G. B., Perego M., Johnson W. C., Burbulys D., Hoch J. A. The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein. EMBO J. 1989 May;8(5):1615–1621. doi: 10.1002/j.1460-2075.1989.tb03546.x. [DOI] [PMC free article] [PubMed] [Google Scholar]