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. 1995 Aug;177(15):4537–4539. doi: 10.1128/jb.177.15.4537-4539.1995

Physical mapping and characterization of a catabolic plasmid from the deep-subsurface bacterium Sphingomonas sp. strain F199.

L C Stillwell 1, S J Thurston 1, R P Schneider 1, M F Romine 1, J K Fredrickson 1, J D Saffer 1
PMCID: PMC177210  PMID: 7635838

Abstract

A supercoiled 180-kb plasmid, pNL1, has been isolated from the deep-subsurface, chemoheterotrophic Sphingomonas sp. strain F199, and a physical map was generated. Analysis of a pNL1-derived cosmid library indicated that catechol 2,3-dioxygenase activity was linked to two distinct regions of the plasmid. Thus, the genes for aromatic catabolism in this Sphingomonas strain are, at least in part, plasmid encoded.

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Selected References

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