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. 1995 Aug;177(15):4540–4543. doi: 10.1128/jb.177.15.4540-4543.1995

Molecular cloning of the pyrE gene from the extreme thermophile Thermus flavus.

V Vonstein 1, S P Johnson 1, H Yu 1, M J Casadaban 1, N C Pagratis 1, J M Weber 1, D C Demirjian 1
PMCID: PMC177211  PMID: 7635839

Abstract

Mutants of the extreme thermophile Thermus flavus in the pyrimidine biosynthetic pathway (Pyr-) were isolated by resistance to 5-fluoroorotic acid. The pyrE gene, which codes for the orotate phosphoribosyltransferase, was cloned by recombination with one of the isolated Pyr- T. flavus mutant strains. It was subcloned by complementation of an Escherichia coli pyrE mutant strain and was sequenced. The deduced polypeptide sequence extends over 183 amino acids. Several independent Pyr- mutations were mapped within the pyrE locus by recombination with fragments of the cloned gene.

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Selected References

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