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. 1995 Aug;177(16):4820–4824. doi: 10.1128/jb.177.16.4820-4824.1995

Activation of the Escherichia coli lacZ promoter by the Klebsiella aerogenes nitrogen assimilation control protein (NAC), a LysR family transcription factor.

P J Pomposiello 1, R A Bender 1
PMCID: PMC177252  PMID: 7642513

Abstract

A chimeric promoter with the nitrogen assimilation control protein binding site from hutUp of Klebsiella aerogenes fused to the lacZ core promoter from Escherichia coli was built and cloned in a lacZ reporter plasmid. This construct showed a 14-fold increase of beta-galactosidase activity upon nitrogen limitation. Primer extension experiments showed that the nitrogen assimilation control protein activates lacZp1 in a position-dependent manner.

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Selected References

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