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. 1995 Sep;177(17):5155–5157. doi: 10.1128/jb.177.17.5155-5157.1995

Identity of the Escherichia coli cls and nov genes.

B E Tropp 1, L Ragolia 1, W Xia 1, W Dowhan 1, R Milkman 1, K E Rudd 1, R Ivanisević 1, D J Savić 1
PMCID: PMC177298  PMID: 7665497

Abstract

cls and nov mutants have similar increased sensitivities to novobiocin and reduced levels of cardiolipin, both of which can be corrected by plasmid-borne copies of either wild-type gene. A comparison of the DNA sequences of both genes further verifies their identity.

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Selected References

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  1. DeChavigny A., Heacock P. N., Dowhan W. Sequence and inactivation of the pss gene of Escherichia coli. Phosphatidylethanolamine may not be essential for cell viability. J Biol Chem. 1991 Mar 15;266(8):5323–5332. [PubMed] [Google Scholar]
  2. Dewitt S K, Adelberg E A. The Occurrence of a Genetic Transposition in a Strain of Escherichia Coli. Genetics. 1962 May;47(5):577–585. doi: 10.1093/genetics/47.5.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gellert M., O'Dea M. H., Itoh T., Tomizawa J. Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4474–4478. doi: 10.1073/pnas.73.12.4474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Heber S., Tropp B. E. Genetic regulation of cardiolipin synthase in Escherichia coli. Biochim Biophys Acta. 1991 Dec 2;1129(1):1–12. doi: 10.1016/0167-4781(91)90206-2. [DOI] [PubMed] [Google Scholar]
  5. Hiraoka S., Matsuzaki H., Shibuya I. Active increase in cardiolipin synthesis in the stationary growth phase and its physiological significance in Escherichia coli. FEBS Lett. 1993 Dec 27;336(2):221–224. doi: 10.1016/0014-5793(93)80807-7. [DOI] [PubMed] [Google Scholar]
  6. Hiraoka S., Nukui K., Uetake N., Ohta A., Shibuya I. Amplification and substantial purification of cardiolipin synthase of Escherichia coli. J Biochem. 1991 Sep;110(3):443–449. doi: 10.1093/oxfordjournals.jbchem.a123600. [DOI] [PubMed] [Google Scholar]
  7. Hwang Y. W., Engel R., Tropp B. E. Correlation of 3,4-dihydroxybutyl 1-phosphonate resistance with a defect in cardiolipin synthesis in Escherichia coli. J Bacteriol. 1984 Mar;157(3):846–856. doi: 10.1128/jb.157.3.846-856.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ivanisevic R., Milić M., Ajdić D., Rakonjac J., Savić D. J. Nucleotide sequence, mutational analysis, transcriptional start site, and product analysis of nov, the gene which affects Escherichia coli K-12 resistance to the gyrase inhibitor novobiocin. J Bacteriol. 1995 Apr;177(7):1766–1771. doi: 10.1128/jb.177.7.1766-1771.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Killian J. A., Koorengevel M. C., Bouwstra J. A., Gooris G., Dowhan W., de Kruijff B. Effect of divalent cations on lipid organization of cardiolipin isolated from Escherichia coli strain AH930. Biochim Biophys Acta. 1994 Jan 19;1189(2):225–232. doi: 10.1016/0005-2736(94)90069-8. [DOI] [PubMed] [Google Scholar]
  10. Milkman R. An Escherichia coli homologue of eukaryotic potassium channel proteins. Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3510–3514. doi: 10.1073/pnas.91.9.3510. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nishijima S., Asami Y., Uetake N., Yamagoe S., Ohta A., Shibuya I. Disruption of the Escherichia coli cls gene responsible for cardiolipin synthesis. J Bacteriol. 1988 Feb;170(2):775–780. doi: 10.1128/jb.170.2.775-780.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ohta A., Obara T., Asami Y., Shibuya I. Molecular cloning of the cls gene responsible for cardiolipin synthesis in Escherichia coli and phenotypic consequences of its amplification. J Bacteriol. 1985 Aug;163(2):506–514. doi: 10.1128/jb.163.2.506-514.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Raetz C. R., Foulds J. Envelope composition and antibiotic hypersensitivity of Escherichia coli mutants defective in phosphatidylserine synthetase. J Biol Chem. 1977 Aug 25;252(16):5911–5915. [PubMed] [Google Scholar]
  14. Ragolia L., Tropp B. E. The effects of phosphoglycerides on Escherichia coli cardiolipin synthase. Biochim Biophys Acta. 1994 Oct 6;1214(3):323–332. doi: 10.1016/0005-2760(94)90080-9. [DOI] [PubMed] [Google Scholar]
  15. Rakonjac J., Milic M., Ajdic-Predic D., Santos D., Ivanisevic R., Savic D. J. nov: a new genetic locus that affects the response of Escherichia coli K-12 to novobiocin. Mol Microbiol. 1992 Jun;6(11):1547–1553. doi: 10.1111/j.1365-2958.1992.tb00876.x. [DOI] [PubMed] [Google Scholar]
  16. Rietveld A. G., Chupin V. V., Koorengevel M. C., Wienk H. L., Dowhan W., de Kruijff B. Regulation of lipid polymorphism is essential for the viability of phosphatidylethanolamine-deficient Escherichia coli cells. J Biol Chem. 1994 Nov 18;269(46):28670–28675. [PubMed] [Google Scholar]
  17. Rietveld A. G., Killian J. A., Dowhan W., de Kruijff B. Polymorphic regulation of membrane phospholipid composition in Escherichia coli. J Biol Chem. 1993 Jun 15;268(17):12427–12433. [PubMed] [Google Scholar]
  18. Shibuya I., Miyazaki C., Ohta A. Alteration of phospholipid composition by combined defects in phosphatidylserine and cardiolipin synthases and physiological consequences in Escherichia coli. J Bacteriol. 1985 Mar;161(3):1086–1092. doi: 10.1128/jb.161.3.1086-1092.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shibuya I., Yamagoe S., Miyazaki C., Matsuzaki H., Ohta A. Biosynthesis of novel acidic phospholipid analogs in Escherichia coli. J Bacteriol. 1985 Feb;161(2):473–477. doi: 10.1128/jb.161.2.473-477.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

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