Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1995 Sep;177(17):5189–5192. doi: 10.1128/jb.177.17.5189-5192.1995

Effects of temperature and of heat shock on the expression and action of the colicin A lysis protein.

D Cavard 1
PMCID: PMC177306  PMID: 7665505

Abstract

At low temperature, the synthesis of the colicin A lysis protein in Escherichia coli was slowed down, and consequently its functioning was retarded. The rates were restored when the bacteria were shifted for 10 min to 42 degrees C, except in an rpoH mutant, suggesting that one or more proteins regulated by sigma 32 is necessary for expression of colicin A lysis protein.

Full Text

The Full Text of this article is available as a PDF (294.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baty D., Lloubès R., Geli V., Lazdunski C., Howard S. P. Extracellular release of colicin A is non-specific. EMBO J. 1987 Aug;6(8):2463–2468. doi: 10.1002/j.1460-2075.1987.tb02526.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brok R. G., Brinkman E., van Boxtel R., Bekkers A. C., Verheij H. M., Tommassen J. Molecular characterization of enterobacterial pldA genes encoding outer membrane phospholipase A. J Bacteriol. 1994 Feb;176(3):861–870. doi: 10.1128/jb.176.3.861-870.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cavard D. Colicin A and colicin E1 lysis proteins differ in their dependence on secA and secY gene products. FEBS Lett. 1992 Feb 17;298(1):84–88. doi: 10.1016/0014-5793(92)80027-e. [DOI] [PubMed] [Google Scholar]
  4. Cavard D., Howard S. P., Lazdunski C. Functioning of the colicin A lysis protein is affected by Triton X-100, divalent cations and EDTA. J Gen Microbiol. 1989 Jun;135(6):1715–1726. doi: 10.1099/00221287-135-6-1715. [DOI] [PubMed] [Google Scholar]
  5. Cavard D., Howard S. P., Lloubes R., Lazdunski C. High-level expression of the colicin A lysis protein. Mol Gen Genet. 1989 Jun;217(2-3):511–519. doi: 10.1007/BF02464925. [DOI] [PubMed] [Google Scholar]
  6. Cavard D., Lazdunski C., Howard S. P. The acylated precursor form of the colicin A lysis protein is a natural substrate of the DegP protease. J Bacteriol. 1989 Nov;171(11):6316–6322. doi: 10.1128/jb.171.11.6316-6322.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cavard D. Truncated mutants of the colicin A lysis protein are acylated and processed when overproduced. FEMS Microbiol Lett. 1994 Apr 1;117(2):169–174. doi: 10.1111/j.1574-6968.1994.tb06760.x. [DOI] [PubMed] [Google Scholar]
  8. Eraso J. M., Weinstock G. M. Anaerobic control of colicin E1 production. J Bacteriol. 1992 Aug;174(15):5101–5109. doi: 10.1128/jb.174.15.5101-5109.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Grossman A. D., Erickson J. W., Gross C. A. The htpR gene product of E. coli is a sigma factor for heat-shock promoters. Cell. 1984 Sep;38(2):383–390. doi: 10.1016/0092-8674(84)90493-8. [DOI] [PubMed] [Google Scholar]
  10. Hayashi S., Wu H. C. Lipoproteins in bacteria. J Bioenerg Biomembr. 1990 Jun;22(3):451–471. doi: 10.1007/BF00763177. [DOI] [PubMed] [Google Scholar]
  11. Lemaux P. G., Herendeen S. L., Bloch P. L., Neidhardt F. C. Transient rates of synthesis of individual polypeptides in E. coli following temperature shifts. Cell. 1978 Mar;13(3):427–434. doi: 10.1016/0092-8674(78)90317-3. [DOI] [PubMed] [Google Scholar]
  12. Lloubes R., Baty D., Lazdunski C. The promoters of the genes for colicin production, release and immunity in the ColA plasmid: effects of convergent transcription and Lex A protein. Nucleic Acids Res. 1986 Mar 25;14(6):2621–2636. doi: 10.1093/nar/14.6.2621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Oliver D. B., Cabelli R. J., Dolan K. M., Jarosik G. P. Azide-resistant mutants of Escherichia coli alter the SecA protein, an azide-sensitive component of the protein export machinery. Proc Natl Acad Sci U S A. 1990 Nov;87(21):8227–8231. doi: 10.1073/pnas.87.21.8227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Oudega B., Mol O., van Ulsen P., Stegehuis F., van der Wal F. J., Luirink J. Escherichia coli SecB, SecA, and SecY proteins are required for expression and membrane insertion of the bacteriocin release protein, a small lipoprotein. J Bacteriol. 1993 Mar;175(5):1543–1547. doi: 10.1128/jb.175.5.1543-1547.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pugsley A. P., Schwartz M. Colicin E2 release: lysis, leakage or secretion? Possible role of a phospholipase. EMBO J. 1984 Oct;3(10):2393–2397. doi: 10.1002/j.1460-2075.1984.tb02145.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Salles B., Weisemann J. M., Weinstock G. M. Temporal control of colicin E1 induction. J Bacteriol. 1987 Nov;169(11):5028–5034. doi: 10.1128/jb.169.11.5028-5034.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tobe T., Ito K., Yura T. Isolation and physical mapping of temperature-sensitive mutants defective in heat-shock induction of proteins in Escherichia coli. Mol Gen Genet. 1984;195(1-2):10–16. doi: 10.1007/BF00332716. [DOI] [PubMed] [Google Scholar]
  18. Wild J., Walter W. A., Gross C. A., Altman E. Accumulation of secretory protein precursors in Escherichia coli induces the heat shock response. J Bacteriol. 1993 Jul;175(13):3992–3997. doi: 10.1128/jb.175.13.3992-3997.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yamamori T., Ito K., Nakamura Y., Yura T. Transient regulation of protein synthesis in Escherichia coli upon shift-up of growth temperature. J Bacteriol. 1978 Jun;134(3):1133–1140. doi: 10.1128/jb.134.3.1133-1140.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES