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. 1995 Sep;177(17):5193–5196. doi: 10.1128/jb.177.17.5193-5196.1995

A partially functional 245-amino-acid internal deletion derivative of Escherichia coli sigma 70.

A Kumar 1, H S Williamson 1, N Fujita 1, A Ishihama 1, R S Hayward 1
PMCID: PMC177307  PMID: 7665506

Abstract

Two hundred forty-five consecutive amino acids of the sigma 70 subunit of Escherichia coli RNA polymerase are not conserved in the homologous protein of Bacillus subtilis. We show that their deletion from a sigma 70-32 hybrid protein caused no severe loss of function in vivo, while sigma 70 itself retained considerable function in vitro.

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Selected References

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  1. Burgess R. R., Travers A. A., Dunn J. J., Bautz E. K. Factor stimulating transcription by RNA polymerase. Nature. 1969 Jan 4;221(5175):43–46. doi: 10.1038/221043a0. [DOI] [PubMed] [Google Scholar]
  2. Burton Z., Burgess R. R., Lin J., Moore D., Holder S., Gross C. A. The nucleotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E coli K12. Nucleic Acids Res. 1981 Jun 25;9(12):2889–2903. doi: 10.1093/nar/9.12.2889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cowing D. W., Gross C. A. Interaction of Escherichia coli RNA polymerase holoenzyme containing sigma 32 with heat shock promoters. DNase I footprinting and methylation protection. J Mol Biol. 1989 Dec 5;210(3):513–520. doi: 10.1016/0022-2836(89)90127-7. [DOI] [PubMed] [Google Scholar]
  4. Gribskov M., Burgess R. R. Sigma factors from E. coli, B. subtilis, phage SP01, and phage T4 are homologous proteins. Nucleic Acids Res. 1986 Aug 26;14(16):6745–6763. doi: 10.1093/nar/14.16.6745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grossman A. D., Erickson J. W., Gross C. A. The htpR gene product of E. coli is a sigma factor for heat-shock promoters. Cell. 1984 Sep;38(2):383–390. doi: 10.1016/0092-8674(84)90493-8. [DOI] [PubMed] [Google Scholar]
  6. Harley C. B., Reynolds R. P. Analysis of E. coli promoter sequences. Nucleic Acids Res. 1987 Mar 11;15(5):2343–2361. doi: 10.1093/nar/15.5.2343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Helmann J. D., Chamberlin M. J. Structure and function of bacterial sigma factors. Annu Rev Biochem. 1988;57:839–872. doi: 10.1146/annurev.bi.57.070188.004203. [DOI] [PubMed] [Google Scholar]
  8. Hu J. C., Gross C. A. Marker rescue with plasmids bearing deletions in rpoD identifies a dispensable part of E. coli sigma factor. Mol Gen Genet. 1983;191(3):492–498. doi: 10.1007/BF00425768. [DOI] [PubMed] [Google Scholar]
  9. Igarashi K., Ishihama A. Bipartite functional map of the E. coli RNA polymerase alpha subunit: involvement of the C-terminal region in transcription activation by cAMP-CRP. Cell. 1991 Jun 14;65(6):1015–1022. doi: 10.1016/0092-8674(91)90553-b. [DOI] [PubMed] [Google Scholar]
  10. Jaehning J. A. Sigma factor relatives in eukaryotes. Science. 1991 Aug 23;253(5022):859–859. doi: 10.1126/science.1876846. [DOI] [PubMed] [Google Scholar]
  11. Kajitani M., Ishihama A. Determination of the promoter strength in the mixed transcription system. II. Promoters of ribosomal RNA, ribosomal protein S1 and recA protein operons from Escherichia coli. Nucleic Acids Res. 1983 Jun 25;11(12):3873–3888. doi: 10.1093/nar/11.12.3873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kumar A., Grimes B., Logan M., Wedgwood S., Williamson H., Hayward R. S. A hybrid sigma subunit directs RNA polymerase to a hybrid promoter in Escherichia coli. J Mol Biol. 1995 Mar 10;246(5):563–571. doi: 10.1016/s0022-2836(05)80105-6. [DOI] [PubMed] [Google Scholar]
  13. Kumar A., Malloch R. A., Fujita N., Smillie D. A., Ishihama A., Hayward R. S. The minus 35-recognition region of Escherichia coli sigma 70 is inessential for initiation of transcription at an "extended minus 10" promoter. J Mol Biol. 1993 Jul 20;232(2):406–418. doi: 10.1006/jmbi.1993.1400. [DOI] [PubMed] [Google Scholar]
  14. Landick R., Vaughn V., Lau E. T., VanBogelen R. A., Erickson J. W., Neidhardt F. C. Nucleotide sequence of the heat shock regulatory gene of E. coli suggests its protein product may be a transcription factor. Cell. 1984 Aug;38(1):175–182. doi: 10.1016/0092-8674(84)90538-5. [DOI] [PubMed] [Google Scholar]
  15. Lesley S. A., Burgess R. R. Characterization of the Escherichia coli transcription factor sigma 70: localization of a region involved in the interaction with core RNA polymerase. Biochemistry. 1989 Sep 19;28(19):7728–7734. doi: 10.1021/bi00445a031. [DOI] [PubMed] [Google Scholar]
  16. Lonetto M. A., Brown K. L., Rudd K. E., Buttner M. J. Analysis of the Streptomyces coelicolor sigE gene reveals the existence of a subfamily of eubacterial RNA polymerase sigma factors involved in the regulation of extracytoplasmic functions. Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7573–7577. doi: 10.1073/pnas.91.16.7573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lonetto M., Gribskov M., Gross C. A. The sigma 70 family: sequence conservation and evolutionary relationships. J Bacteriol. 1992 Jun;174(12):3843–3849. doi: 10.1128/jb.174.12.3843-3849.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nomura T., Fujita N., Ishihama A. Promoter selectivity of E. coli RNA polymerase: analysis of the promoter system of convergently-transcribed dnaQ-rnh genes. Nucleic Acids Res. 1985 Nov 11;13(21):7647–7661. doi: 10.1093/nar/13.21.7647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nomura T., Fujita N., Ishihama A. Promoter selectivity of Escherichia coli RNA polymerase: alteration by fMet-tRNAfMet. Nucleic Acids Res. 1986 Sep 11;14(17):6857–6870. doi: 10.1093/nar/14.17.6857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rossi J. J., Soberon X., Marumoto Y., McMahon J., Itakura K. Biological expression of an Escherichia coli consensus sequence promoter and some mutant derivatives. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3203–3207. doi: 10.1073/pnas.80.11.3203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shorenstein R. G., Losick R. Comparative size and properties of the sigma subunits of ribonucleic acid polymerase from Bacillus subtilis and Escherichia coli. J Biol Chem. 1973 Sep 10;248(17):6170–6173. [PubMed] [Google Scholar]
  22. Travers A. A., Burgessrr Cyclic re-use of the RNA polymerase sigma factor. Nature. 1969 May 10;222(5193):537–540. doi: 10.1038/222537a0. [DOI] [PubMed] [Google Scholar]
  23. Waldburger C., Susskind M. M. Probing the informational content of Escherichia coli sigma 70 region 2.3 by combinatorial cassette mutagenesis. J Mol Biol. 1994 Feb 4;235(5):1489–1500. doi: 10.1006/jmbi.1994.1103. [DOI] [PubMed] [Google Scholar]
  24. Zou C., Fujita N., Igarashi K., Ishihama A. Mapping the cAMP receptor protein contact site on the alpha subunit of Escherichia coli RNA polymerase. Mol Microbiol. 1992 Sep;6(18):2599–2605. doi: 10.1111/j.1365-2958.1992.tb01437.x. [DOI] [PubMed] [Google Scholar]

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