Abstract
A physical restriction map of the chromosome of the green sulfur bacterium Chlorobium tepidum was generated by determining the order of the fragments obtained after digestion with the restriction endonucleases XbaI and PacI and subsequent separation of the fragments by pulsed-field gel electrophoresis. The size of the chromosome is estimated to be 2.1 Mb. Fifteen genes and operons, mainly encoding proteins involved in photosynthesis, have been placed on this map by hybridization to fragments obtained after single- and double-restriction digestions.
Full Text
The Full Text of this article is available as a PDF (366.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bancroft I., Wolk C. P., Oren E. V. Physical and genetic maps of the genome of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. J Bacteriol. 1989 Nov;171(11):5940–5948. doi: 10.1128/jb.171.11.5940-5948.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blankenship R. E. Origin and early evolution of photosynthesis. Photosynth Res. 1992;33:91–111. [PubMed] [Google Scholar]
- Büttner M., Xie D. L., Nelson H., Pinther W., Hauska G., Nelson N. Photosynthetic reaction center genes in green sulfur bacteria and in photosystem 1 are related. Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):8135–8139. doi: 10.1073/pnas.89.17.8135. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carlson C. R., Grønstad A., Kolstø A. B. Physical maps of the genomes of three Bacillus cereus strains. J Bacteriol. 1992 Jun;174(11):3750–3756. doi: 10.1128/jb.174.11.3750-3756.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chen X., Widger W. R. Physical genome map of the unicellular cyanobacterium Synechococcus sp. strain PCC 7002. J Bacteriol. 1993 Aug;175(16):5106–5116. doi: 10.1128/jb.175.16.5106-5116.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cole S. T., Saint Girons I. Bacterial genomics. FEMS Microbiol Rev. 1994 Jun;14(2):139–160. doi: 10.1111/j.1574-6976.1994.tb00084.x. [DOI] [PubMed] [Google Scholar]
- Fonstein M., Haselkorn R. Chromosomal structure of Rhodobacter capsulatus strain SB1003: cosmid encyclopedia and high-resolution physical and genetic map. Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2522–2526. doi: 10.1073/pnas.90.6.2522. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fox G. E., Stackebrandt E., Hespell R. B., Gibson J., Maniloff J., Dyer T. A., Wolfe R. S., Balch W. E., Tanner R. S., Magrum L. J. The phylogeny of prokaryotes. Science. 1980 Jul 25;209(4455):457–463. doi: 10.1126/science.6771870. [DOI] [PubMed] [Google Scholar]
- Majumdar D., Avissar Y. J., Wyche J. H., Beale S. I. Structure and expression of the Chlorobium vibrioforme hemA gene. Arch Microbiol. 1991;156(4):281–289. doi: 10.1007/BF00262999. [DOI] [PubMed] [Google Scholar]
- Okkels J. S., Kjaer B., Hansson O., Svendsen I., Møller B. L., Scheller H. V. A membrane-bound monoheme cytochrome c551 of a novel type is the immediate electron donor to P840 of the Chlorobium vibrioforme photosynthetic reaction center complex. J Biol Chem. 1992 Oct 15;267(29):21139–21145. [PubMed] [Google Scholar]
- Sirevåg R., Ormerod J. G. Carbon dioxide fixation in green sulphur bacteria. Biochem J. 1970 Nov;120(2):399–408. doi: 10.1042/bj1200399. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Suwanto A., Kaplan S. Physical and genetic mapping of the Rhodobacter sphaeroides 2.4.1 genome: presence of two unique circular chromosomes. J Bacteriol. 1989 Nov;171(11):5850–5859. doi: 10.1128/jb.171.11.5850-5859.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woese C. R., Stackebrandt E., Macke T. J., Fox G. E. A phylogenetic definition of the major eubacterial taxa. Syst Appl Microbiol. 1985;6:143–151. doi: 10.1016/s0723-2020(85)80047-3. [DOI] [PubMed] [Google Scholar]