Figure 5 .

Gastrin induced degradation of inhibitor κB (IκB)-α. MKGR26 cells and MKN-neo cells were treated with gastrin for 0, 5, 15, or 30 minutes and cell lysates were obtained. Proteins were size fractionated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, and then transferred to polyvinylidine difluoride membranes. Membranes were reacted with rabbit antihuman-IκB-α antibody, incubated with peroxidase conjugated goat antirabbit IgG, and peroxidase was detected with an enhanced chemiluminescence system. Quantitated signals were determined by densitometry.