Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1995 Oct;177(20):5860–5864. doi: 10.1128/jb.177.20.5860-5864.1995

Fructosebisphosphatase isoenzymes of the chemoautotroph Xanthobacter flavus.

E R van den Bergh 1, T A van der Kooij 1, L Dijkhuizen 1, W G Meijer 1
PMCID: PMC177410  PMID: 7592335

Abstract

Xanthobacter flavus employs two fructosebisphosphatase (FBPase)-sedoheptulosebisphosphatase (SBPase) enzymes. One of these is constitutively expressed and has a high FBPase-to-SBPase ratio. The alternative enzyme, which is encoded by cbbF, is induced during autotrophic growth. The cbbF gene was expressed in Escherichia coli, and the FBPase was purified to homogeneity. The purified enzyme has a specific FBPase activity of 114 mumol/min/mg of protein, a Michaelis constant for fructosebisphosphate of 3 microM, and a low FBPase-to-SBPase ratio. CbbF was activated by ATP and inhibited by Ca2+.

Full Text

The Full Text of this article is available as a PDF (213.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdelal A. T., Schlegel H. G. Purification and regulatory properties of fructose 1,6-diphosphatase from Hydrogenomonas eutropha. J Bacteriol. 1974 Oct;120(1):304–310. doi: 10.1128/jb.120.1.304-310.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  3. Chardot T., Meunier J. C. Properties of oxidized and reduced spinach (Spinacia oleracea) chloroplast fructose-1,6-bisphosphatase activated by various agents. Biochem J. 1991 Sep 15;278(Pt 3):787–791. doi: 10.1042/bj2780787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Charles S. A., Halliwell B. Action of calcium ions on spinach (Spinacia oleracea) chloroplast fructose bisphosphatase and other enzymes of the Calvin cycle. Biochem J. 1980 Jun 15;188(3):775–779. doi: 10.1042/bj1880775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Falcone D. L., Tabita F. R. Complementation analysis and regulation of CO2 fixation gene expression in a ribulose 1,5-bisphosphate carboxylase-oxygenase deletion strain of Rhodospirillum rubrum. J Bacteriol. 1993 Aug;175(16):5066–5077. doi: 10.1128/jb.175.16.5066-5077.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gibson J. L., Tabita F. R. Different molecular forms of D-ribulose-1,5-bisphosphate carboxylase from Rhodopseudomonas sphaeroides. J Biol Chem. 1977 Feb 10;252(3):943–949. [PubMed] [Google Scholar]
  7. Gibson J. L., Tabita F. R. Localization and mapping of CO2 fixation genes within two gene clusters in Rhodobacter sphaeroides. J Bacteriol. 1988 May;170(5):2153–2158. doi: 10.1128/jb.170.5.2153-2158.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Huppe H. C., de Lamotte-Guéry F., Jacquot J-P, Buchanan B. B. The ferredoxin-thioredoxin system of a green alga, Chlamydomonas reinhardtii: identification and characterization of thioredoxins and ferredoxin-thioredoxin reductase components. Planta. 1990;180:341–351. [PubMed] [Google Scholar]
  9. Irani M. H., Maitra P. K. Properties of Escherichia coli mutants deficient in enzymes of glycolysis. J Bacteriol. 1977 Nov;132(2):398–410. doi: 10.1128/jb.132.2.398-410.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jarrell K. F., Faguy D., Hebert A. M., Kalmokoff M. L. A general method of isolating high molecular weight DNA from methanogenic archaea (archaebacteria). Can J Microbiol. 1992 Jan;38(1):65–68. doi: 10.1139/m92-010. [DOI] [PubMed] [Google Scholar]
  11. Kusian B., Yoo J. G., Bednarski R., Bowien B. The Calvin cycle enzyme pentose-5-phosphate 3-epimerase is encoded within the cfx operons of the chemoautotroph Alcaligenes eutrophus. J Bacteriol. 1992 Nov;174(22):7337–7344. doi: 10.1128/jb.174.22.7337-7344.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ladror U. S., Latshaw S. P., Marcus F. Spinach cytosolic fructose-1,6-bisphosphatase. Purification, enzyme properties and structural comparisons. Eur J Biochem. 1990 Apr 20;189(1):89–94. doi: 10.1111/j.1432-1033.1990.tb15463.x. [DOI] [PubMed] [Google Scholar]
  13. Lehmicke L. G., Lidstrom M. E. Organization of genes necessary for growth of the hydrogen-methanol autotroph Xanthobacter sp. strain H4-14 on hydrogen and carbon dioxide. J Bacteriol. 1985 Jun;162(3):1244–1249. doi: 10.1128/jb.162.3.1244-1249.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Levering P. R., van Dijken J. P., Veenhius M., Harder W. Arthrobacter P1, a fast growing versatile methylotroph with amine oxidase as a key enzyme in the metabolism of methylated amines. Arch Microbiol. 1981 Mar;129(1):72–80. doi: 10.1007/BF00417184. [DOI] [PubMed] [Google Scholar]
  15. Marcus F., Harrsch P. B. Amino acid sequence of spinach chloroplast fructose-1,6-bisphosphatase. Arch Biochem Biophys. 1990 May 15;279(1):151–157. doi: 10.1016/0003-9861(90)90475-e. [DOI] [PubMed] [Google Scholar]
  16. Meijer W. G., Arnberg A. C., Enequist H. G., Terpstra P., Lidstrom M. E., Dijkhuizen L. Identification and organization of carbon dioxide fixation genes in Xanthobacter flavus H4-14. Mol Gen Genet. 1991 Feb;225(2):320–330. doi: 10.1007/BF00269865. [DOI] [PubMed] [Google Scholar]
  17. Meijer W. G., Croes L. M., Jenni B., Lehmicke L. G., Lidstrom M. E., Dijkhuizen L. Characterization of Xanthobacter strains H4-14 and 25a and enzyme profiles after growth under autotrophic and heterotrophic conditions. Arch Microbiol. 1990;153(4):360–367. doi: 10.1007/BF00249006. [DOI] [PubMed] [Google Scholar]
  18. Meijer W. G., Enequist H. G., Terpstra P., Dijkhuizen L. Nucleotide sequences of the genes encoding fructosebisphosphatase and phosphoribulokinase from Xanthobacter flavus H4-14. J Gen Microbiol. 1990 Nov;136(11):2225–2230. doi: 10.1099/00221287-136-11-2225. [DOI] [PubMed] [Google Scholar]
  19. Meijer W. G. The Calvin cycle enzyme phosphoglycerate kinase of Xanthobacter flavus required for autotrophic CO2 fixation is not encoded by the cbb operon. J Bacteriol. 1994 Oct;176(19):6120–6126. doi: 10.1128/jb.176.19.6120-6126.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tabita F. R., Gibson J. L., Bowien B., Dijkhuizen L., Meijer W. G. Uniform designation for genes of the Calvin-Benson-Bassham reductive pentose phosphate pathway of bacteria. FEMS Microbiol Lett. 1992 Dec 1;78(2-3):107–110. doi: 10.1111/j.1574-6968.1992.tb05551.x. [DOI] [PubMed] [Google Scholar]
  21. Windhövel U., Bowien B. On the operon structure of the cfx gene clusters in Alcaligenes eutrophus. Arch Microbiol. 1990;154(1):85–91. doi: 10.1007/BF00249183. [DOI] [PubMed] [Google Scholar]
  22. Zimmermann G., Kelly G. J., Latzko E. Efficient purification and molecular properties of spinach chloroplast fructose 1,6-bisphosphatase. Eur J Biochem. 1976 Nov 15;70(2):361–367. doi: 10.1111/j.1432-1033.1976.tb11025.x. [DOI] [PubMed] [Google Scholar]
  23. Zimmermann G., Kelly G. J., Latzko E. Purification and properties of spinach leaf cytoplasmic fructose-1,6-bisphosphatase. J Biol Chem. 1978 Sep 10;253(17):5952–5956. [PubMed] [Google Scholar]
  24. van den Bergh E. R., Dijkhuizen L., Meijer W. G. CbbR, a LysR-type transcriptional activator, is required for expression of the autotrophic CO2 fixation enzymes of Xanthobacter flavus. J Bacteriol. 1993 Oct;175(19):6097–6104. doi: 10.1128/jb.175.19.6097-6104.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES