Abstract
We carried out studies with Escherichia coli to determine the site at which the methylation-independent pathways for taxis to oxygen and to sugars of the phosphoenolpyruvate:sugar phosphotransferase transport system converge with the methylation-dependent chemotaxis pathways. Using genetic reconstitution of the pathways in a null strain, we determined that all pathways examined required the products of the genes cheA, cheW, and cheY. Thus, we conclude that both the methylation-independent and methylation-dependent pathways converge at CheA, the histidine kinase product of cheA.
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Selected References
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- Block S. M., Segall J. E., Berg H. C. Adaptation kinetics in bacterial chemotaxis. J Bacteriol. 1983 Apr;154(1):312–323. doi: 10.1128/jb.154.1.312-323.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bourret R. B., Borkovich K. A., Simon M. I. Signal transduction pathways involving protein phosphorylation in prokaryotes. Annu Rev Biochem. 1991;60:401–441. doi: 10.1146/annurev.bi.60.070191.002153. [DOI] [PubMed] [Google Scholar]
- Conley M. P., Wolfe A. J., Blair D. F., Berg H. C. Both CheA and CheW are required for reconstitution of chemotactic signaling in Escherichia coli. J Bacteriol. 1989 Sep;171(9):5190–5193. doi: 10.1128/jb.171.9.5190-5193.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dang C. V., Niwano M., Ryu J., Taylor B. L. Inversion of aerotactic response in Escherichia coli deficient in cheB protein methylesterase. J Bacteriol. 1986 Apr;166(1):275–280. doi: 10.1128/jb.166.1.275-280.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuo S. C., Koshland D. E., Jr Roles of cheY and cheZ gene products in controlling flagellar rotation in bacterial chemotaxis of Escherichia coli. J Bacteriol. 1987 Mar;169(3):1307–1314. doi: 10.1128/jb.169.3.1307-1314.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Niwano M., Taylor B. L. Novel sensory adaptation mechanism in bacterial chemotaxis to oxygen and phosphotransferase substrates. Proc Natl Acad Sci U S A. 1982 Jan;79(1):11–15. doi: 10.1073/pnas.79.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shioi J., Taylor B. L. Oxygen taxis and proton motive force in Salmonella typhimurium. J Biol Chem. 1984 Sep 10;259(17):10983–10988. [PubMed] [Google Scholar]
- Shioi J., Tribhuwan R. C., Berg S. T., Taylor B. L. Signal transduction in chemotaxis to oxygen in Escherichia coli and Salmonella typhimurium. J Bacteriol. 1988 Dec;170(12):5507–5511. doi: 10.1128/jb.170.12.5507-5511.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Springer W. R., Koshland D. E., Jr Identification of a protein methyltransferase as the cheR gene product in the bacterial sensing system. Proc Natl Acad Sci U S A. 1977 Feb;74(2):533–537. doi: 10.1073/pnas.74.2.533. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor B. L. Role of proton motive force in sensory transduction in bacteria. Annu Rev Microbiol. 1983;37:551–573. doi: 10.1146/annurev.mi.37.100183.003003. [DOI] [PubMed] [Google Scholar]
- Van Der Werf P., Koshland D. E., Jr Identification of a gamma-glutamyl methyl ester in bacterial membrane protein involved in chemotaxis. J Biol Chem. 1977 Apr 25;252(8):2793–2795. [PubMed] [Google Scholar]
- Wolfe A. J., Conley M. P., Kramer T. J., Berg H. C. Reconstitution of signaling in bacterial chemotaxis. J Bacteriol. 1987 May;169(5):1878–1885. doi: 10.1128/jb.169.5.1878-1885.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]