Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1995 Oct;177(20):6015–6017. doi: 10.1128/jb.177.20.6015-6017.1995

Distance between alleles as a determinant of linkage in natural transformation of Acinetobacter calcoaceticus.

D U Kloos 1, A A DiMarco 1, D A Elsemore 1, K N Timmis 1, L N Ornston 1
PMCID: PMC177435  PMID: 7592360

Abstract

Cotransformation frequencies of 16, 39, 51, and 60% were observed when donor alleles were separated by distances of 9.2, 7.4, 6.3, and 5.1 kb, respectively, in donor Acinetobacter calcoaceticus DNA. A different and unexpected pattern was observed when the distance between recipient alleles was reduced from 9.2 to 5.1 kb. Ligation of unlinked chromosomal DNA fragments allowed them to be linked genetically through natural transformation.

Full Text

The Full Text of this article is available as a PDF (159.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Averhoff B., Gregg-Jolly L., Elsemore D., Ornston L. N. Genetic analysis of supraoperonic clustering by use of natural transformation in Acinetobacter calcoaceticus. J Bacteriol. 1992 Jan;174(1):200–204. doi: 10.1128/jb.174.1.200-204.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BERNS K. I., THOMAS C. A., Jr ISOLATION OF HIGH MOLECULAR WEIGHT DNA FROM HEMOPHILUS INFLUENZAE. J Mol Biol. 1965 Mar;11:476–490. doi: 10.1016/s0022-2836(65)80004-3. [DOI] [PubMed] [Google Scholar]
  3. DiMarco A. A., Averhoff B. A., Kim E. E., Ornston L. N. Evolutionary divergence of pobA, the structural gene encoding p-hydroxybenzoate hydroxylase in an Acinetobacter calcoaceticus strain well-suited for genetic analysis. Gene. 1993 Mar 15;125(1):25–33. doi: 10.1016/0378-1119(93)90741-k. [DOI] [PubMed] [Google Scholar]
  4. DiMarco A. A., Averhoff B., Ornston L. N. Identification of the transcriptional activator pobR and characterization of its role in the expression of pobA, the structural gene for p-hydroxybenzoate hydroxylase in Acinetobacter calcoaceticus. J Bacteriol. 1993 Jul;175(14):4499–4506. doi: 10.1128/jb.175.14.4499-4506.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doten R. C., Gregg L. A., Ornston L. N. Influence of the catBCE sequence on the phenotypic reversion of a pcaE mutation in Acinetobacter calcoaceticus. J Bacteriol. 1987 Jul;169(7):3175–3180. doi: 10.1128/jb.169.7.3175-3180.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Elsemore D. A., Ornston L. N. The pca-pob supraoperonic cluster of Acinetobacter calcoaceticus contains quiA, the structural gene for quinate-shikimate dehydrogenase. J Bacteriol. 1994 Dec;176(24):7659–7666. doi: 10.1128/jb.176.24.7659-7666.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Elsemore D. A., Ornston L. N. Unusual ancestry of dehydratases associated with quinate catabolism in Acinetobacter calcoaceticus. J Bacteriol. 1995 Oct;177(20):5971–5978. doi: 10.1128/jb.177.20.5971-5978.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gerischer U., Ornston L. N. Spontaneous mutations in pcaH and -G, structural genes for protocatechuate 3,4-dioxygenase in Acinetobacter calcoaceticus. J Bacteriol. 1995 Mar;177(5):1336–1347. doi: 10.1128/jb.177.5.1336-1347.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gregg-Jolly L. A., Ornston L. N. Recovery of DNA from the Acinetobacter calcoaceticus chromosome by gap repair. J Bacteriol. 1990 Oct;172(10):6169–6172. doi: 10.1128/jb.172.10.6169-6172.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hartnett G. B., Averhoff B., Ornston L. N. Selection of Acinetobacter calcoaceticus mutants deficient in the p-hydroxybenzoate hydroxylase gene (pobA), a member of a supraoperonic cluster. J Bacteriol. 1990 Oct;172(10):6160–6161. doi: 10.1128/jb.172.10.6160-6161.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Juni E., Janik A. Transformation of Acinetobacter calco-aceticus (Bacterium anitratum). J Bacteriol. 1969 Apr;98(1):281–288. doi: 10.1128/jb.98.1.281-288.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kowalchuk G. A., Gregg-Jolly L. A., Ornston L. N. Nucleotide sequences transferred by gene conversion in the bacterium Acinetobacter calcoaceticus. Gene. 1995 Feb 3;153(1):111–115. doi: 10.1016/0378-1119(94)00760-p. [DOI] [PubMed] [Google Scholar]
  13. Kowalchuk G. A., Hartnett G. B., Benson A., Houghton J. E., Ngai K. L., Ornston L. N. Contrasting patterns of evolutionary divergence within the Acinetobacter calcoaceticus pca operon. Gene. 1994 Aug 19;146(1):23–30. doi: 10.1016/0378-1119(94)90829-x. [DOI] [PubMed] [Google Scholar]
  14. Neidle E. L., Hartnett C., Bonitz S., Ornston L. N. DNA sequence of the Acinetobacter calcoaceticus catechol 1,2-dioxygenase I structural gene catA: evidence for evolutionary divergence of intradiol dioxygenases by acquisition of DNA sequence repetitions. J Bacteriol. 1988 Oct;170(10):4874–4880. doi: 10.1128/jb.170.10.4874-4880.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Neidle E. L., Ornston L. N. Cloning and expression of Acinetobacter calcoaceticus catechol 1,2-dioxygenase structural gene catA in Escherichia coli. J Bacteriol. 1986 Nov;168(2):815–820. doi: 10.1128/jb.168.2.815-820.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES