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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1995 Nov;177(21):6294–6296. doi: 10.1128/jb.177.21.6294-6296.1995

Immunoelectron microscopic localization of one of the spore germination proteins, GerAB, in Bacillus subtilis spores.

Y Sakae 1, Y Yasuda 1, K Tochikubo 1
PMCID: PMC177473  PMID: 7592398

Abstract

Ultrastructural localization of GerAB, one of the proteins of Bacillus subtilis spores related to L-alanine-initiated germination, was investigated by immunoelectron microscopy with antipeptide (residues 61 to 80 of GerAB) antiserum and a colloidal gold-immunoglobulin G complex. Immunogold particles were visualized in the boundary region between the cortex and coat of dormant spores, and they were broadly dispersed into the cortex region after germination.

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