Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1995 Nov;177(21):6309–6312. doi: 10.1128/jb.177.21.6309-6312.1995

Antigenic determinants of the membrane-bound hydrogenase in Alcaligenes eutrophus are exposed toward the periplasm.

K Eismann 1, K Mlejnek 1, D Zipprich 1, M Hoppert 1, H Gerberding 1, F Mayer 1
PMCID: PMC177477  PMID: 7592402

Abstract

Electron microscopic immunogold labeling experiments were performed with ultrathin sections of plasmolyzed cells of Alcaligenes eutrophus and "whole-mount" samples of spheroplasts and protoplasts. They demonstrated that antigenic determinants of the membrane-bound hydrogenase are exposed, at the outside of the cytoplasmic membrane, to the periplasm.

Full Text

The Full Text of this article is available as a PDF (1.1 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bendayan M., Roth J., Perrelet A., Orci L. Quantitative immunocytochemical localization of pancreatic secretory proteins in subcellular compartments of the rat acinar cell. J Histochem Cytochem. 1980 Feb;28(2):149–160. doi: 10.1177/28.2.7354212. [DOI] [PubMed] [Google Scholar]
  2. Braks I. J., Hoppert M., Roge S., Mayer F. Structural aspects and immunolocalization of the F420-reducing and non-F420-reducing hydrogenases from Methanobacterium thermoautotrophicum Marburg. J Bacteriol. 1994 Dec;176(24):7677–7687. doi: 10.1128/jb.176.24.7677-7687.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eiffert H., Schlott T., Hoppert M., Lotter H., Thomssen R. Identification of an endoflagellar associated protein in Borrelia burgdorferi. J Med Microbiol. 1992 Mar;36(3):209–214. doi: 10.1099/00222615-36-3-209. [DOI] [PubMed] [Google Scholar]
  4. Friedrich B., Heine E., Finck A., Friedrich C. G. Nickel requirement for active hydrogenase formation in Alcaligenes eutrophus. J Bacteriol. 1981 Mar;145(3):1144–1149. doi: 10.1128/jb.145.3.1144-1149.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Friedrich B., Kortlüke C., Hogrefe C., Eberz G., Silber B., Warrelmann J. Genetics of hydrogenase from aerobic lithoautotrophic bacteria. Biochimie. 1986 Jan;68(1):133–145. doi: 10.1016/s0300-9084(86)81078-1. [DOI] [PubMed] [Google Scholar]
  6. Friedrich C. G. Depression of hydrogenase during limitation of electron donors and derepression of ribulosebisphosphate carboxylase during carbon limitation of Alcaligenes eutrophus. J Bacteriol. 1982 Jan;149(1):203–210. doi: 10.1128/jb.149.1.203-210.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gerberding H., Mayer F. Interaction and compartmentalization of the components of bacterial enzyme systems involved in cell energetics. Z Naturforsch C. 1993 Jul-Aug;48(7-8):535–541. doi: 10.1515/znc-1993-7-801. [DOI] [PubMed] [Google Scholar]
  8. Gerberding H., Mayer F. Structural organization of the membrane-bound hydrogenase isolated from Alcaligenes eutrophus as revealed by electron microscopy. FEMS Microbiol Lett. 1989 Jul 15;51(1):159–163. doi: 10.1016/0378-1097(89)90500-4. [DOI] [PubMed] [Google Scholar]
  9. Hogrefe C., Römermann D., Friedrich B. Alcaligenes eutrophus hydrogenase genes (Hox). J Bacteriol. 1984 Apr;158(1):43–48. doi: 10.1128/jb.158.1.43-48.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jacoby G. H., Young K. D. Cell cycle-independent lysis of Escherichia coli by cefsulodin, an inhibitor of penicillin-binding proteins 1a and 1b. J Bacteriol. 1991 Jan;173(1):1–5. doi: 10.1128/jb.173.1.1-5.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kortlüke C., Friedrich B. Maturation of membrane-bound hydrogenase of Alcaligenes eutrophus H16. J Bacteriol. 1992 Oct;174(19):6290–6293. doi: 10.1128/jb.174.19.6290-6293.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kortlüke C., Horstmann K., Schwartz E., Rohde M., Binsack R., Friedrich B. A gene complex coding for the membrane-bound hydrogenase of Alcaligenes eutrophus H16. J Bacteriol. 1992 Oct;174(19):6277–6289. doi: 10.1128/jb.174.19.6277-6289.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mayer F., Rohde M., Salzmann M., Jussofie A., Gottschalk G. The methanoreductosome: a high-molecular-weight enzyme complex in the methanogenic bacterium strain Gö1 that contains components of the methylreductase system. J Bacteriol. 1988 Apr;170(4):1438–1444. doi: 10.1128/jb.170.4.1438-1444.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rohde M., Fürstenau U., Mayer F., Przybyla A. E., Peck H. D., Jr, Le Gall J., Choi E. S., Menon N. K. Localization of membrane-associated (NiFe) and (NiFeSe) hydrogenases of Desulfovibrio vulgaris using immunoelectron microscopic procedures. Eur J Biochem. 1990 Jul 31;191(2):389–396. doi: 10.1111/j.1432-1033.1990.tb19134.x. [DOI] [PubMed] [Google Scholar]
  15. Roth J., Bendayan M., Carlemalm E., Villiger W., Garavito M. Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue. J Histochem Cytochem. 1981 May;29(5):663–671. doi: 10.1177/29.5.6166664. [DOI] [PubMed] [Google Scholar]
  16. Roth J., Bendayan M., Orci L. Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. J Histochem Cytochem. 1978 Dec;26(12):1074–1081. doi: 10.1177/26.12.366014. [DOI] [PubMed] [Google Scholar]
  17. SCHLEGEL H. G., KALTWASSER H., GOTTSCHALK G. [A submersion method for culture of hydrogen-oxidizing bacteria: growth physiological studies]. Arch Mikrobiol. 1961;38:209–222. [PubMed] [Google Scholar]
  18. Schink B., Schlegel H. G. The membrane-bound hydrogenase of Alcaligenes eutrophus. I. Solubilization, purification, and biochemical properties. Biochim Biophys Acta. 1979 Apr 12;567(2):315–324. doi: 10.1016/0005-2744(79)90117-7. [DOI] [PubMed] [Google Scholar]
  19. Schneider K., Schlegel H. G. Purification and properties of soluble hydrogenase from Alcaligenes eutrophus H 16. Biochim Biophys Acta. 1976 Nov 8;452(1):66–80. doi: 10.1016/0005-2744(76)90058-9. [DOI] [PubMed] [Google Scholar]
  20. Valentine R. C., Shapiro B. M., Stadtman E. R. Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coli. Biochemistry. 1968 Jun;7(6):2143–2152. doi: 10.1021/bi00846a017. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES