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. 1995 Nov;177(21):6313–6315. doi: 10.1128/jb.177.21.6313-6315.1995

The regulatory C proteins from different restriction-modification systems can cross-complement.

C L Ives 1, A Sohail 1, J E Brooks 1
PMCID: PMC177478  PMID: 7592403

Abstract

The BamHI restriction-modification system contains a third gene, bamHIC, which positively regulates bamHIR. Similar small genes from other systems were tested in vivo for their ability to cross-complement. C.BamHI protein was identified, purified, and used to raise polyclonal antibodies. Attempts to detect other C proteins in cell extracts by cross-reactivity with C.BamHI antibodies proved unsuccessful.

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Selected References

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  1. Bougueleret L., Schwarzstein M., Tsugita A., Zabeau M. Characterization of the genes coding for the Eco RV restriction and modification system of Escherichia coli. Nucleic Acids Res. 1984 Apr 25;12(8):3659–3676. doi: 10.1093/nar/12.8.3659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brooks J. E., Benner J. S., Heiter D. F., Silber K. R., Sznyter L. A., Jager-Quinton T., Moran L. S., Slatko B. E., Wilson G. G., Nwankwo D. O. Cloning the BamHI restriction modification system. Nucleic Acids Res. 1989 Feb 11;17(3):979–997. doi: 10.1093/nar/17.3.979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brooks J. E., Nathan P. D., Landry D., Sznyter L. A., Waite-Rees P., Ives C. L., Moran L. S., Slatko B. E., Benner J. S. Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts. Nucleic Acids Res. 1991 Feb 25;19(4):841–850. doi: 10.1093/nar/19.4.841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Heidmann S., Seifert W., Kessler C., Domdey H. Cloning, characterization and heterologous expression of the SmaI restriction-modification system. Nucleic Acids Res. 1989 Dec 11;17(23):9783–9796. doi: 10.1093/nar/17.23.9783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ives C. L., Nathan P. D., Brooks J. E. Regulation of the BamHI restriction-modification system by a small intergenic open reading frame, bamHIC, in both Escherichia coli and Bacillus subtilis. J Bacteriol. 1992 Nov;174(22):7194–7201. doi: 10.1128/jb.174.22.7194-7201.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jack W. E., Greenough L., Dorner L. F., Xu S. Y., Strzelecka T., Aggarwal A. K., Schildkraut I. Overexpression, purification and crystallization of BamHI endonuclease. Nucleic Acids Res. 1991 Apr 25;19(8):1825–1829. doi: 10.1093/nar/19.8.1825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nathan P. D., Brooks J. E. Characterization of clones of the BamHI methyltransferase gene. Gene. 1988 Dec 25;74(1):35–36. doi: 10.1016/0378-1119(88)90244-2. [DOI] [PubMed] [Google Scholar]
  9. Roberts R. J., Wilson G. A., Young F. E. Recognition sequence of specific endonuclease BamH.I from Bacillus amyloliquefaciens H. Nature. 1977 Jan 6;265(5589):82–84. doi: 10.1038/265082a0. [DOI] [PubMed] [Google Scholar]
  10. Sutton M. R., Fall R. R., Nervi A. M., Alberts A. W., Vagelos P. R., Bradshaw R. A. Amino acid sequence of Escherichia coli biotin carboxyl carrier protein (9100). J Biol Chem. 1977 Jun 10;252(11):3934–3940. [PubMed] [Google Scholar]
  11. Tao T., Blumenthal R. M. Sequence and characterization of pvuIIR, the PvuII endonuclease gene, and of pvuIIC, its regulatory gene. J Bacteriol. 1992 May;174(10):3395–3398. doi: 10.1128/jb.174.10.3395-3398.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tao T., Bourne J. C., Blumenthal R. M. A family of regulatory genes associated with type II restriction-modification systems. J Bacteriol. 1991 Feb;173(4):1367–1375. doi: 10.1128/jb.173.4.1367-1375.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Theriault G., Roy P. H., Howard K. A., Benner J. S., Brooks J. E., Waters A. F., Gingeras T. R. Nucleotide sequence of the PaeR7 restriction/modification system and partial characterization of its protein products. Nucleic Acids Res. 1985 Dec 9;13(23):8441–8461. doi: 10.1093/nar/13.23.8441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Waite-Rees P. A., Keating C. J., Moran L. S., Slatko B. E., Hornstra L. J., Benner J. S. Characterization and expression of the Escherichia coli Mrr restriction system. J Bacteriol. 1991 Aug;173(16):5207–5219. doi: 10.1128/jb.173.16.5207-5219.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Way J. C., Davis M. A., Morisato D., Roberts D. E., Kleckner N. New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition. Gene. 1984 Dec;32(3):369–379. doi: 10.1016/0378-1119(84)90012-x. [DOI] [PubMed] [Google Scholar]
  17. Wilson G. A., Young F. E. Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H. J Mol Biol. 1975 Sep 5;97(1):123–125. doi: 10.1016/s0022-2836(75)80028-3. [DOI] [PubMed] [Google Scholar]
  18. Wilson G. G. Organization of restriction-modification systems. Nucleic Acids Res. 1991 May 25;19(10):2539–2566. doi: 10.1093/nar/19.10.2539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

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