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Detection of protease activated receptor 4 (PAR4) in rat colon by reverse transcription-polymerase chain reaction. Lanes 1 and 2, colon tissues where PAR4 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) primers were amplified; a specific 463 bp fragment corresponds to PAR4 while GAPDH showed an expected amplified fragment of 306 bp. Lane 3 shows omission of PAR4 primer.
