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. 1995 Nov;177(22):6492–6498. doi: 10.1128/jb.177.22.6492-6498.1995

Physical map of the linear chromosome of Streptomyces griseus.

A Lezhava 1, T Mizukami 1, T Kajitani 1, D Kameoka 1, M Redenbach 1, H Shinkawa 1, O Nimi 1, H Kinashi 1
PMCID: PMC177500  PMID: 7592425

Abstract

The chromosomal DNA of Streptomyces griseus 2247 (a derivative of strain IFO3237) was digested with several restriction endonucleases and analyzed by pulsed-field gel electrophoresis (PFGE). Digestion with AseI and DraI gave 15 and 9 fragments, respectively, the total sizes of which were 7.8 Mb. All the AseI and DraI fragments were aligned on a linear chromosome map by using linking plasmids and cosmids. PFGE analysis of the intact chromosome also showed a linear DNA band of about 8 Mb. Detailed physical maps of both terminal regions were constructed; they revealed the presence of a 24-kb terminal inverted repeat on each end. PFGE analysis with and without proteinase K treatment suggested that each end of the chromosome carries a protein molecule.

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Selected References

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