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. 1995 Nov;177(22):6666–6669. doi: 10.1128/jb.177.22.6666-6669.1995

Molecular cloning with a pMEA300-derived shuttle vector and characterization of the Amycolatopsis methanolica prephenate dehydratase gene.

J W Vrijbloed 1, J van Hylckama Vlieg 1, N M van der Put 1, G I Hessels 1, L Dijkhuizen 1
PMCID: PMC177523  PMID: 7592448

Abstract

An efficient restriction barrier for methylated DNA in the actinomycete Amycolatopsis methanolica could be avoided by using a nonmethylating Escherichia coli strain for DNA isolations. The A. methanolica prephenate dehydratase gene was cloned from a gene bank in a pMEA300-derived shuttle vector in E. coli and characterized.

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Selected References

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