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. 1995 Nov;177(22):6679–6683. doi: 10.1128/jb.177.22.6679-6683.1995

Expression of kinA and accumulation of sigma H at the onset of sporulation in Bacillus subtilis.

K Asai 1, F Kawamura 1, H Yoshikawa 1, H Takahashi 1
PMCID: PMC177527  PMID: 7592452

Abstract

Induction of the Bacillus subtilis kinA gene, which codes for a major kinase of the phosphorelay pathway, required the spo0H gene, coding for the sigma H protein, but not the genes spo0A, spo0B, and spo0F at the onset of sporulation. Also, the levels of sigma H in spo0A, spo0B, and spo0F mutants were increased at the onset of sporulation, though induction of spo0H transcription in all of these mutants was appreciably inhibited. In addition, kinA expression was almost completely eliminated in a medium supplemented with excess glucose and glutamine, even though the usual stationary-phase-associated increase in sigma H was observed under these conditions.

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Selected References

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  1. Antoniewski C., Savelli B., Stragier P. The spoIIJ gene, which regulates early developmental steps in Bacillus subtilis, belongs to a class of environmentally responsive genes. J Bacteriol. 1990 Jan;172(1):86–93. doi: 10.1128/jb.172.1.86-93.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burbulys D., Trach K. A., Hoch J. A. Initiation of sporulation in B. subtilis is controlled by a multicomponent phosphorelay. Cell. 1991 Feb 8;64(3):545–552. doi: 10.1016/0092-8674(91)90238-t. [DOI] [PubMed] [Google Scholar]
  3. Chibazakura T., Kawamura F., Asai K., Takahashi H. Effects of spo0 mutations on spo0A promoter switching at the initiation of sporulation in Bacillus subtilis. J Bacteriol. 1995 Aug;177(15):4520–4523. doi: 10.1128/jb.177.15.4520-4523.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chibazakura T., Kawamura F., Takahashi H. Differential regulation of spo0A transcription in Bacillus subtilis: glucose represses promoter switching at the initiation of sporulation. J Bacteriol. 1991 Apr;173(8):2625–2632. doi: 10.1128/jb.173.8.2625-2632.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dubnau E., Weir J., Nair G., Carter L., 3rd, Moran C., Jr, Smith I. Bacillus sporulation gene spo0H codes for sigma 30 (sigma H). J Bacteriol. 1988 Mar;170(3):1054–1062. doi: 10.1128/jb.170.3.1054-1062.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ferrari F. A., Trach K., LeCoq D., Spence J., Ferrari E., Hoch J. A. Characterization of the spo0A locus and its deduced product. Proc Natl Acad Sci U S A. 1985 May;82(9):2647–2651. doi: 10.1073/pnas.82.9.2647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Frisby D., Zuber P. Mutations in pts cause catabolite-resistant sporulation and altered regulation of spo0H in Bacillus subtilis. J Bacteriol. 1994 May;176(9):2587–2595. doi: 10.1128/jb.176.9.2587-2595.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grossman A. D., Losick R. Extracellular control of spore formation in Bacillus subtilis. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4369–4373. doi: 10.1073/pnas.85.12.4369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Healy J., Weir J., Smith I., Losick R. Post-transcriptional control of a sporulation regulatory gene encoding transcription factor sigma H in Bacillus subtilis. Mol Microbiol. 1991 Feb;5(2):477–487. doi: 10.1111/j.1365-2958.1991.tb02131.x. [DOI] [PubMed] [Google Scholar]
  10. Kobayashi K., Shoji K., Shimizu T., Nakano K., Sato T., Kobayashi Y. Analysis of a suppressor mutation ssb (kinC) of sur0B20 (spo0A) mutation in Bacillus subtilis reveals that kinC encodes a histidine protein kinase. J Bacteriol. 1995 Jan;177(1):176–182. doi: 10.1128/jb.177.1.176-182.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kudoh J., Ikeuchi T., Kurahashi K. Nucleotide sequences of the sporulation gene spo0A and its mutant genes of Bacillus subtilis. Proc Natl Acad Sci U S A. 1985 May;82(9):2665–2668. doi: 10.1073/pnas.82.9.2665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LeDeaux J. R., Grossman A. D. Isolation and characterization of kinC, a gene that encodes a sensor kinase homologous to the sporulation sensor kinases KinA and KinB in Bacillus subtilis. J Bacteriol. 1995 Jan;177(1):166–175. doi: 10.1128/jb.177.1.166-175.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mandic-Mulec I., Gaur N., Bai U., Smith I. Sin, a stage-specific repressor of cellular differentiation. J Bacteriol. 1992 Jun;174(11):3561–3569. doi: 10.1128/jb.174.11.3561-3569.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Perego M., Cole S. P., Burbulys D., Trach K., Hoch J. A. Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis. J Bacteriol. 1989 Nov;171(11):6187–6196. doi: 10.1128/jb.171.11.6187-6196.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Perego M., Spiegelman G. B., Hoch J. A. Structure of the gene for the transition state regulator, abrB: regulator synthesis is controlled by the spo0A sporulation gene in Bacillus subtilis. Mol Microbiol. 1988 Nov;2(6):689–699. doi: 10.1111/j.1365-2958.1988.tb00079.x. [DOI] [PubMed] [Google Scholar]
  16. Piggot P. J., Coote J. G. Genetic aspects of bacterial endospore formation. Bacteriol Rev. 1976 Dec;40(4):908–962. doi: 10.1128/br.40.4.908-962.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Predich M., Nair G., Smith I. Bacillus subtilis early sporulation genes kinA, spo0F, and spo0A are transcribed by the RNA polymerase containing sigma H. J Bacteriol. 1992 May;174(9):2771–2778. doi: 10.1128/jb.174.9.2771-2778.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Satola S., Kirchman P. A., Moran C. P., Jr Spo0A binds to a promoter used by sigma A RNA polymerase during sporulation in Bacillus subtilis. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4533–4537. doi: 10.1073/pnas.88.10.4533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schaeffer P., Millet J., Aubert J. P. Catabolic repression of bacterial sporulation. Proc Natl Acad Sci U S A. 1965 Sep;54(3):704–711. doi: 10.1073/pnas.54.3.704. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stock J. B., Ninfa A. J., Stock A. M. Protein phosphorylation and regulation of adaptive responses in bacteria. Microbiol Rev. 1989 Dec;53(4):450–490. doi: 10.1128/mr.53.4.450-490.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Strauch M. A., Hoch J. A. Transition-state regulators: sentinels of Bacillus subtilis post-exponential gene expression. Mol Microbiol. 1993 Feb;7(3):337–342. doi: 10.1111/j.1365-2958.1993.tb01125.x. [DOI] [PubMed] [Google Scholar]
  22. Strauch M. A., Spiegelman G. B., Perego M., Johnson W. C., Burbulys D., Hoch J. A. The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein. EMBO J. 1989 May;8(5):1615–1621. doi: 10.1002/j.1460-2075.1989.tb03546.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Strauch M. A., Wu J. J., Jonas R. H., Hoch J. A. A positive feedback loop controls transcription of the spoOF gene, a component of the sporulation phosphorelay in Bacillus subtilis. Mol Microbiol. 1993 Mar;7(6):967–974. doi: 10.1111/j.1365-2958.1993.tb01188.x. [DOI] [PubMed] [Google Scholar]
  24. Strauch M., Webb V., Spiegelman G., Hoch J. A. The SpoOA protein of Bacillus subtilis is a repressor of the abrB gene. Proc Natl Acad Sci U S A. 1990 Mar;87(5):1801–1805. doi: 10.1073/pnas.87.5.1801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Trach K. A., Hoch J. A. Multisensory activation of the phosphorelay initiating sporulation in Bacillus subtilis: identification and sequence of the protein kinase of the alternate pathway. Mol Microbiol. 1993 Apr;8(1):69–79. doi: 10.1111/j.1365-2958.1993.tb01204.x. [DOI] [PubMed] [Google Scholar]
  26. Trach K., Burbulys D., Strauch M., Wu J. J., Dhillon N., Jonas R., Hanstein C., Kallio P., Perego M., Bird T. Control of the initiation of sporulation in Bacillus subtilis by a phosphorelay. Res Microbiol. 1991 Sep-Oct;142(7-8):815–823. doi: 10.1016/0923-2508(91)90060-n. [DOI] [PubMed] [Google Scholar]
  27. Weir J., Predich M., Dubnau E., Nair G., Smith I. Regulation of spo0H, a gene coding for the Bacillus subtilis sigma H factor. J Bacteriol. 1991 Jan;173(2):521–529. doi: 10.1128/jb.173.2.521-529.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Yamashita S., Kawamura F., Yoshikawa H., Takahashi H., Kobayashi Y., Saito H. Dissection of the expression signals of the spoA gene of Bacillus subtilis: glucose represses sporulation-specific expression. J Gen Microbiol. 1989 May;135(5):1335–1345. doi: 10.1099/00221287-135-5-1335. [DOI] [PubMed] [Google Scholar]
  29. Yamashita S., Yoshikawa H., Kawamura F., Takahashi H., Yamamoto T., Kobayashi Y., Saito H. The effect of spo0 mutations on the expression of spo0A- and spo0F-lacZ fusions. Mol Gen Genet. 1986 Oct;205(1):28–33. doi: 10.1007/BF02428029. [DOI] [PubMed] [Google Scholar]
  30. York K., Kenney T. J., Satola S., Moran C. P., Jr, Poth H., Youngman P. Spo0A controls the sigma A-dependent activation of Bacillus subtilis sporulation-specific transcription unit spoIIE. J Bacteriol. 1992 Apr;174(8):2648–2658. doi: 10.1128/jb.174.8.2648-2658.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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