Abstract
The genome of Bartonella bacilliformis was shown to be a single circular DNA molecule of about 1,600 kbp having six NotI, four SfiI, and two CeuI sites. A physical map of the DNA was constructed by contour-clamped homogeneous electric field pulsed-field gel electrophoresis of DNA restriction fragments. rRNA operons, the invasion-associated locus, and a flagellin gene were located on the map by hybridization.
Full Text
The Full Text of this article is available as a PDF (328.9 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barton B. M., Harding G. P., Zuccarelli A. J. A general method for detecting and sizing large plasmids. Anal Biochem. 1995 Apr 10;226(2):235–240. doi: 10.1006/abio.1995.1220. [DOI] [PubMed] [Google Scholar]
- Beard P., Morrow J. F., Berg P. Cleavage of circular, superhelical simian virus 40 DNA to a linear duplex by S1 nuclease. J Virol. 1973 Dec;12(6):1303–1313. doi: 10.1128/jvi.12.6.1303-1313.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Birtles R. J., Harrison T. G., Fry N. K., Saunders N. A., Taylor A. G. Taxonomic considerations of Bartonella bacilliformis based on phylogenetic and phenotypic characteristics. FEMS Microbiol Lett. 1991 Oct 1;67(2):187–191. doi: 10.1016/0378-1097(91)90352-b. [DOI] [PubMed] [Google Scholar]
- Brenner D. J., O'Connor S. P., Hollis D. G., Weaver R. E., Steigerwalt A. G. Molecular characterization and proposal of a neotype strain for Bartonella bacilliformis. J Clin Microbiol. 1991 Jul;29(7):1299–1302. doi: 10.1128/jcm.29.7.1299-1302.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Daly J. S., Worthington M. G., Brenner D. J., Moss C. W., Hollis D. G., Weyant R. S., Steigerwalt A. G., Weaver R. E., Daneshvar M. I., O'Connor S. P. Rochalimaea elizabethae sp. nov. isolated from a patient with endocarditis. J Clin Microbiol. 1993 Apr;31(4):872–881. doi: 10.1128/jcm.31.4.872-881.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kreier J. P., Ristic M. The biology of hemotrophic bacteria. Annu Rev Microbiol. 1981;35:325–338. doi: 10.1146/annurev.mi.35.100181.001545. [DOI] [PubMed] [Google Scholar]
- Liu S. L., Hessel A., Sanderson K. E. Genomic mapping with I-Ceu I, an intron-encoded endonuclease specific for genes for ribosomal RNA, in Salmonella spp., Escherichia coli, and other bacteria. Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6874–6878. doi: 10.1073/pnas.90.14.6874. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mernaugh G., Ihler G. M. Deformation factor: an extracellular protein synthesized by Bartonella bacilliformis that deforms erythrocyte membranes. Infect Immun. 1992 Mar;60(3):937–943. doi: 10.1128/iai.60.3.937-943.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Minnick M. F. Nucleotide sequence of the 5S ribosomal RNA gene of Bartonella bacilliformis. Nucleic Acids Res. 1993 Feb 25;21(4):1036–1036. doi: 10.1093/nar/21.4.1036. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mitchell S. J., Minnick M. F. Characterization of a two-gene locus from Bartonella bacilliformis associated with the ability to invade human erythrocytes. Infect Immun. 1995 Apr;63(4):1552–1562. doi: 10.1128/iai.63.4.1552-1562.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Connor S. P., Dorsch M., Steigerwalt A. G., Brenner D. J., Stackebrandt E. 16S rRNA sequences of Bartonella bacilliformis and cat scratch disease bacillus reveal phylogenetic relationships with the alpha-2 subgroup of the class Proteobacteria. J Clin Microbiol. 1991 Oct;29(10):2144–2150. doi: 10.1128/jcm.29.10.2144-2150.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]