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. 1973 Aug;48(4):629–639. doi: 10.1111/j.1476-5381.1973.tb08250.x

The contribution of prostaglandin production to contractions of the isolated uterus of the rat

J R Vane, K I Williams
PMCID: PMC1776150  PMID: 4788206

Abstract

1. Indomethacin and meclofenamate, both of which are potent inhibitors of prostaglandin synthetase, antagonized the contractor effects of oxytocin on the isolated uterus from the non-pregnant rat. Contractions induced by acetylcholine or prostaglandin F2a were not antagonized.

2. Uteri from rats 17-22 days pregnant exhibited intermittent spontaneous contractions when used as isolated preparations. They also released prostaglandin-like activity (mainly similar to F2a) into the bathing fluid. Both the prostaglandin release and the uterine activity were abolished by indomethacin. Activity could be restored by addition of low concentrations of prostaglandin E2 or F2a.

3. The release of prostaglandin F2a-like activity by the uteri increased dramatically on the expected day of delivery (day 22).

4. The results add force to the hypothesis that the spontaneous activity of some isolated organs is due to an intramural prostaglandin generation, and that increased uterine prostaglandin production contributes to the expulsion of the foetus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aiken J. W. Aspirin and indomethacin prolong parturition in rats: evidence that prostaglandins contribute to expulsion of fetus. Nature. 1972 Nov 3;240(5375):21–25. doi: 10.1038/240021a0. [DOI] [PubMed] [Google Scholar]
  2. Bergström S., Carlson L. A., Weeks J. R. The prostaglandins: a family of biologically active lipids. Pharmacol Rev. 1968 Mar;20(1):1–48. [PubMed] [Google Scholar]
  3. Brummer H. C. Further studies on the interaction between prostaglandins and syntocinon on the isolated pregnant human myometrium. J Obstet Gynaecol Br Commonw. 1972 Jun;79(6):526–530. doi: 10.1111/j.1471-0528.1972.tb14195.x. [DOI] [PubMed] [Google Scholar]
  4. Brummer H. C. Interaction of E prostaglandins and syntocinon on the pregnant human myometrium. J Obstet Gynaecol Br Commonw. 1971 Apr;78(4):305–309. doi: 10.1111/j.1471-0528.1971.tb00277.x. [DOI] [PubMed] [Google Scholar]
  5. Challis J. R., Harrison F. A., Heap R. B., Horton E. W., Poyser N. L. A possible role of oestrogens in the stimulation of prostaglandin F 2 output at the time of parturition in a sheep. J Reprod Fertil. 1972 Sep;30(3):485–488. doi: 10.1530/jrf.0.0300485. [DOI] [PubMed] [Google Scholar]
  6. Chester R., Dukes M., Slater S. R., Walpole A. L. Delay of parturition in the rat by anti-inflammatory agents which inhibit the biosynthesis of prostaglandins. Nature. 1972 Nov 3;240(5375):37–38. doi: 10.1038/240037a0. [DOI] [PubMed] [Google Scholar]
  7. Clegg P. C., Hall W. J., Pickles V. R. The action of ketonic prostaglandins on the guinea-pig myometrium. J Physiol. 1966 Mar;183(1):123–144. doi: 10.1113/jphysiol.1966.sp007855. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eckenfels A., Vane J. R. Prostaglandins, oxygen tension and smooth muscle tone. Br J Pharmacol. 1972 Jul;45(3):451–462. doi: 10.1111/j.1476-5381.1972.tb08101.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ferreira S. H., Herman A., Vane J. R. Proceedings: Prostaglandin generation maintains the smooth muscle tone of the rabbit isolated jejunum. Br J Pharmacol. 1972 Feb;44(2):328P–329P. [PMC free article] [PubMed] [Google Scholar]
  10. Ferreira S. H., Moncada S., Vane J. R. Indomethacin and aspirin abolish prostaglandin release from the spleen. Nat New Biol. 1971 Jun 23;231(25):237–239. doi: 10.1038/newbio231237a0. [DOI] [PubMed] [Google Scholar]
  11. Ferreira S. H., Moncada S., Vane J. R. Some effects of inhibiting endogenous prostaglandin formation on the responses of the cat spleen. Br J Pharmacol. 1973 Jan;47(1):48–58. doi: 10.1111/j.1476-5381.1973.tb08157.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Flower R., Gryglewski R., Herbaczyńska-Cedro K., Vane J. R. Effects of anti-inflammatory drugs on prostaglandin biosynthesis. Nat New Biol. 1972 Jul 26;238(82):104–106. doi: 10.1038/newbio238104a0. [DOI] [PubMed] [Google Scholar]
  13. GADDUM J. H. The technique of superfusion. Br J Pharmacol Chemother. 1953 Sep;8(3):321–326. doi: 10.1111/j.1476-5381.1953.tb00801.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. GREEN K., SAMUELSSON B. PROSTAGLANDINS AND RELATED FACTORS: XIX. THIN-LAYER CHROMATOGRAPHY OF PROSTAGLANDINS. J Lipid Res. 1964 Jan;5:117–120. [PubMed] [Google Scholar]
  15. Gilmore N., Vane J. R., Wyllie J. H. Prostaglandins released by the spleen. Nature. 1968 Jun 22;218(5147):1135–1140. doi: 10.1038/2181135a0. [DOI] [PubMed] [Google Scholar]
  16. Karim S. M. Appearance of prostaglandin F2-alpha in human blood during labour. Br Med J. 1968 Dec 7;4(5631):618–621. doi: 10.1136/bmj.4.5631.618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Karim S. M., Devlin J. Prostaglandin content of amniotic fluid during pregnancy and labour. J Obstet Gynaecol Br Commonw. 1967 Apr;74(2):230–234. doi: 10.1111/j.1471-0528.1967.tb14866.x. [DOI] [PubMed] [Google Scholar]
  18. McGiff J. C., Crowshaw K., Terragno N. A., Lonigro A. J. Release of a prostaglandin-like substance into renal venous blood in response to angiotensin II. Circ Res. 1970 Jul;27(1 Suppl 1):121–130. [PubMed] [Google Scholar]
  19. McGiff J. C., Terragno N. A., Malik K. U., Lonigro A. J. Release of a prostaglandin E-like substance from canine kidney by bradykinin. Circ Res. 1972 Jul;31(1):36–43. doi: 10.1161/01.res.31.1.36. [DOI] [PubMed] [Google Scholar]
  20. Piper P., Vane J. The release of prostaglandins from lung and other tissues. Ann N Y Acad Sci. 1971 Apr 30;180:363–385. doi: 10.1111/j.1749-6632.1971.tb53205.x. [DOI] [PubMed] [Google Scholar]
  21. Posner J., Paterson G. The release of prostaglandin E2 from the bovine iris. Br J Pharmacol. 1970 Sep;40(1):163P–164P. [PMC free article] [PubMed] [Google Scholar]
  22. Poyser N. L., Horton E. W., Thompson C. J., Los M. Identification of prostaglandin F2-alpha released by distension of guinea-pig uterus in vitro. Nature. 1971 Apr 23;230(5295):526–528. doi: 10.1038/230526a0. [DOI] [PubMed] [Google Scholar]
  23. Smith J. B., Willis A. L. Aspirin selectively inhibits prostaglandin production in human platelets. Nat New Biol. 1971 Jun 23;231(25):235–237. doi: 10.1038/newbio231235a0. [DOI] [PubMed] [Google Scholar]
  24. Vane J. R. Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nat New Biol. 1971 Jun 23;231(25):232–235. doi: 10.1038/newbio231232a0. [DOI] [PubMed] [Google Scholar]
  25. Vane J. R., Williams K. I. A sensitive method for the assay of oxytocin in blood. Br J Pharmacol. 1970 Feb;38(2):444P–445P. [PMC free article] [PubMed] [Google Scholar]
  26. Vane J. R., Williams K. I. Prostaglandin production contributes to the contractions of the rat isolated uterus. Br J Pharmacol. 1972 May;45(1):146P–146P. [PMC free article] [PubMed] [Google Scholar]
  27. Williams K. I. Prostaglandin synthesis by the pregnant rat uterus at term and its possible relevance in parturition. Br J Pharmacol. 1973 Mar;47(3):628P–629P. [PMC free article] [PubMed] [Google Scholar]

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