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. 1973 Oct;49(2):258–266. doi: 10.1111/j.1476-5381.1973.tb08371.x

[3H]-adenine nucleotide and [3H]-noradrenaline release evoked by electrical field stimulation, perivascular nerve stimulation and nicotine from the taenia of the guinea-pig caecum

M Kuchii, J T Miyahara, S Shibata
PMCID: PMC1776372  PMID: 4793467

Abstract

1. The release of [3H]-noradrenaline and adenine nucleotide evoked by electrical field stimulation (20-60 V, 30 Hz), perivascular nerve stimulation (20-80 V, 60 Hz) and nicotine (10, 100 μM) was studied in the taenia of the guinea-pig caecum under various conditions.

2. Electrical stimulation at high intensity (60 V) caused the release of [3H]-adenine nucleotide; however, the inhibitory action of electrical stimulation was proportional to [3H]-noradrenaline release.

3. The intensity of the inhibitory effect of stimulation of the perivascular nerves was directly related to [3H]-noradrenaline release and not associated with the release of [3H]-nucleotide.

4. Cold storage for more than 8 days, cooling (19° C) or tetrodotoxin treatment (1 μg/ml) abolished the inhibitory responses to electrical stimulation and to nicotine. After these treatments, nicotine and electrical stimulation elicited only contractions; the release of [3H]-noradrenaline, but not that of [3H]-adenine nucleotide, was inhibited.

5. The dissociation of the inhibitory effects of electrical stimulation and nicotine from [3H]-nucleotide release does not support the hypothesis that ATP or a related nucleotide is the humoral transmitter of the non-adrenergic inhibition in the taenia of the guinea-pig caecum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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