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. 1973 Sep;49(1):74–85. doi: 10.1111/j.1476-5381.1973.tb08269.x

The influence of histamine and other vasoactive substances on contractile responses of cat skeletal muscle to acetylcholine

A J Block, E Reit
PMCID: PMC1776400  PMID: 4362593

Abstract

1. The effect of vasoactive substances, chiefly histamine, on contractions of cat skeletal muscle evoked by acetylcholine has been studied. For this purpose all of these agents were administered intra-arterially to anterior tibialis, chiefly, and also to soleus and external rectus muscles in vivo.

2. Under these conditions, histamine itself never altered the resting tension of the muscle, but always enhanced the increase in tension produced by acetylcholine injected shortly afterward.

3. On the anterior tibialis muscle, it was determined that the threshold potentiating dose of histamine was 0·001 μg/kg, and that maximal enhancement occurred when 0·1 μg/kg was injected 15 s before fixed, submaximal doses of acetylcholine. Injected 15 s before graded doses of acetylcholine, histamine 0·1 μg/kg shifted the acetylcholine dose-response curves approximately 1·5 log units to the left.

4. Under similar experimental conditions the vasodilator, bradykinin, 0·001-1 μg/kg, and procedures which produce a local increase in nutritive capillary blood flow also enhanced acetylcholine-evoked contractile responses of the anterior tibialis, whereas the vasoconstrictor, angiotensin, 0·001-1 μg/kg, inhibited them.

5. The potentiating effect of histamine was antagonized by mepyramine, but was undiminished by denervation of the muscle either acutely or for 4 days.

6. Histamine failed to enhance responses of the muscle to electrical stimulation of either the muscle, directly, or its motor nerve.

7. Histamine also had no effect upon acetylcholine-evoked responses of the isolated external rectus muscle in vitro.

8. It is suggested that the potentiating effect of histamine in vivo is due to its vasodilator action within the muscles which, by relaxing arterioles and precapillary sphincters, could allow more of the blood-borne acetylcholine to reach more of its receptors on the muscle fibres in a given time.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown G. L., Harvey A. M. Neuro-muscular transmission in the extrinsic muscles of the eye. J Physiol. 1941 Mar 25;99(3):379–399. doi: 10.1113/jphysiol.1941.sp003910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COBBOLD A., FOLKOW B., KJELLMER I., MELLANDER S. Nervous and local chemical control of pre-capillary sphincters in skeletal muscle as measured by changes in filtration coefficient. Acta Physiol Scand. 1963 Jan-Feb;57:180–192. doi: 10.1111/j.1748-1716.1963.tb02584.x. [DOI] [PubMed] [Google Scholar]
  3. Cervoni P., Reit E. Interaction between angiotensin and bloodborne exogenous norepinephrine on the cat nictitating membrane in vivo. J Pharmacol Exp Ther. 1971 Jun;177(3):633–640. [PubMed] [Google Scholar]
  4. Dale H. H., Gaddum J. H. Reactions of denervated voluntary muscle, and their bearing on the mode of action of parasympathetic and related nerves. J Physiol. 1930 Sep 18;70(2):109–144. doi: 10.1113/jphysiol.1930.sp002682. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Giarman N. J., Reit E. An effect of histamine on the nictitating membrane of the cat: potentiation of the actions of adrenaline, noradrenaline and acetylcholine. Br J Pharmacol Chemother. 1967 Feb;29(2):168–180. doi: 10.1111/j.1476-5381.1967.tb01950.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HILTON S. M. Experiments on the post contraction hyperaemia of skeletal muscle. J Physiol. 1953 Apr 28;120(1-2):230–245. doi: 10.1113/jphysiol.1953.sp004888. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HIRVONEN L., KOROBKIN M., SONNENSCHEIN R. R., WRIGHT D. L. DEPRESSION OF CONTRATILE FORCE OF SKELETAL MUSCLE BY INTRA-ARTERIAL VASODILATOR DRUGS. Circ Res. 1964 Jun;14:525–535. doi: 10.1161/01.res.14.6.525. [DOI] [PubMed] [Google Scholar]
  8. KJELLMER I., ODELRAM H. THE EFFECT OF SOME PHYSIOLOGICAL VASODILATORS ON THE VASCULAR BED OF SKELETAL MUSCLE. Acta Physiol Scand. 1965 Jan-Feb;63:94–102. doi: 10.1111/j.1748-1716.1965.tb04046.x. [DOI] [PubMed] [Google Scholar]
  9. KJELLMER I. THE EFFECT OF EXERCISE ON THE VASCULAR BED OF SKELETAL MUSCLE. Acta Physiol Scand. 1964 Sep-Oct;62:18–30. doi: 10.1111/j.1748-1716.1964.tb03947.x. [DOI] [PubMed] [Google Scholar]
  10. Krogh A. The supply of oxygen to the tissues and the regulation of the capillary circulation. J Physiol. 1919 May 20;52(6):457–474. doi: 10.1113/jphysiol.1919.sp001844. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MACMILLAN W. H. The effects of histamine on skeletal muscle contraction. Arch Int Pharmacodyn Ther. 1956 Oct 1;108(1):19–26. [PubMed] [Google Scholar]
  12. MACMILLAN W. H., VANE J. R. The effects of histamine on the plasma potassium levels of cats. J Pharmacol Exp Ther. 1956 Oct;118(2):182–192. [PubMed] [Google Scholar]
  13. Mellander S., Johansson B. Control of resistance, exchange, and capacitance functions in the peripheral circulation. Pharmacol Rev. 1968 Sep;20(3):117–196. [PubMed] [Google Scholar]
  14. Sonnenschein R. R., Wright D. L., Mellander S. Effects of vasodilators on filtration coefficient and distal arterial pressure in muscle. Am J Physiol. 1967 Sep;213(3):706–710. doi: 10.1152/ajplegacy.1967.213.3.706. [DOI] [PubMed] [Google Scholar]

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