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. 1974 Jan;50(1):137–143. doi: 10.1111/j.1476-5381.1974.tb09601.x

Inhibition by cromoglycate of histamine release from rat peritoneal mast cells induced by mixtures of dextran, phosphatidyl serine and calcium ions

LG Garland, JL Mongar
PMCID: PMC1776568  PMID: 4132696

Abstract

1 Dextran releases histamine from rat peritoneal mast cells in the presence but not in the absence of phosphatidyl serine (PS). With PS (10 μg/ml) present the effect of dextran was concentration-dependent in the range of 0.2-6 mg/ml.

2 PS releases little histamine on its own but mixed with dextran (6 mg/ml) produces a graded effect in the range of 0.3-10 μg/ml.

3 The combination of dextran and PS releases no histamine in a medium containing less than 0.1 mM calcium. As the calcium concentration is increased, release occurs and a maximum is reached at calcium 1 mM; at higher concentrations release falls off sharply.

4 Histamine release by the combination of dextran (6 mg/ml), PS (10 μg/ml) and calcium (1.8 mM) is inhibited by cromoglycate in the same range (1-30 μM) that inhibits anaphylactic histamine release from rat peritoneal cells.

5 Inhibition by cromoglycate occurred with all dextran concentrations tested (0.2-15 mg/ml) but high concentrations of PS (30 and 100 μg/ml) overcame the effect of cromoglycate (2 and 10 μM). Calcium concentrations above 1 mM augmented inhibition by cromoglycate 2 and 10 μM.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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