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. 1996 Jan;178(1):314–316. doi: 10.1128/jb.178.1.314-316.1996

Identification of an anaerobically induced phosphoenolpyruvate-dependent fructose-specific phosphotransferase system and evidence for the Embden-Meyerhof glycolytic pathway in the heterofermentative bacterium Lactobacillus brevis.

M H Saier Jr 1, J J Ye 1, S Klinke 1, E Nino 1
PMCID: PMC177658  PMID: 8550437

Abstract

Heterofermentative gram-positive bacteria are believed to metabolize sugars exclusively via the pentose phosphoketolase pathway following uptake via sugar:cation symport. Here we show that anaerobic growth of one such bacterium, Lactobacillus brevis, in the presence of fructose induces the synthesis of a phosphotransferase system and glycolytic enzymes that allow fructose to be metabolized via the Embden-Meyerhof pathway.

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Selected References

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  1. DeMOSS R. D., BARD R. C., GUNSALUS I. C. The mechanism of the heterolactic fermentation; a new route of ethanol formation. J Bacteriol. 1951 Oct;62(4):499–511. doi: 10.1128/jb.62.4.499-511.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fleischmann R. D., Adams M. D., White O., Clayton R. A., Kirkness E. F., Kerlavage A. R., Bult C. J., Tomb J. F., Dougherty B. A., Merrick J. M. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science. 1995 Jul 28;269(5223):496–512. doi: 10.1126/science.7542800. [DOI] [PubMed] [Google Scholar]
  3. Kornberg H. L. Fructose transport by Escherichia coli. Philos Trans R Soc Lond B Biol Sci. 1990 Jan 30;326(1236):505–513. doi: 10.1098/rstb.1990.0028. [DOI] [PubMed] [Google Scholar]
  4. Mitchell W. J., Reizer J., Herring C., Hoischen C., Saier M. H., Jr Identification of a phosphoenolpyruvate:fructose phosphotransferase system (fructose-1-phosphate forming) in Listeria monocytogenes. J Bacteriol. 1993 May;175(9):2758–2761. doi: 10.1128/jb.175.9.2758-2761.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Reizer J., Peterkofsky A., Romano A. H. Evidence for the presence of heat-stable protein (HPr) and ATP-dependent HPr kinase in heterofermentative lactobacilli lacking phosphoenolpyruvate:glycose phosphotransferase activity. Proc Natl Acad Sci U S A. 1988 Apr;85(7):2041–2045. doi: 10.1073/pnas.85.7.2041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Romano A. H., Brino G., Peterkofsky A., Reizer J. Regulation of beta-galactoside transport and accumulation in heterofermentative lactic acid bacteria. J Bacteriol. 1987 Dec;169(12):5589–5596. doi: 10.1128/jb.169.12.5589-5596.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Romano A. H., Trifone J. D., Brustolon M. Distribution of the phosphoenolpyruvate:glucose phosphotransferase system in fermentative bacteria. J Bacteriol. 1979 Jul;139(1):93–97. doi: 10.1128/jb.139.1.93-97.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Saier M. H., Jr, Chauvaux S., Deutscher J., Reizer J., Ye J. J. Protein phosphorylation and regulation of carbon metabolism in gram-negative versus gram-positive bacteria. Trends Biochem Sci. 1995 Jul;20(7):267–271. doi: 10.1016/s0968-0004(00)89041-6. [DOI] [PubMed] [Google Scholar]
  9. Saier M. H., Jr, Feucht B. U., Mora W. K. Sugar phosphate: sugar transphosphorylation and exchange group translocation catalyzed by the enzyme 11 complexes of the bacterial phosphoenolpyruvate: sugar phosphotransferase system. J Biol Chem. 1977 Dec 25;252(24):8899–8907. [PubMed] [Google Scholar]
  10. Saier M. H., Jr, Reizer J. Proposed uniform nomenclature for the proteins and protein domains of the bacterial phosphoenolpyruvate: sugar phosphotransferase system. J Bacteriol. 1992 Mar;174(5):1433–1438. doi: 10.1128/jb.174.5.1433-1438.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Saier M. H., Jr, Reizer J. The bacterial phosphotransferase system: new frontiers 30 years later. Mol Microbiol. 1994 Sep;13(5):755–764. doi: 10.1111/j.1365-2958.1994.tb00468.x. [DOI] [PubMed] [Google Scholar]
  12. Titgemeyer F., Walkenhorst J., Cui X., Reizer J., Saier M. H., Jr Proteins of the phosphoenolpyruvate:sugar phosphotransferase system in Streptomyces: possible involvement in the regulation of antibiotic production. Res Microbiol. 1994 Feb;145(2):89–92. doi: 10.1016/0923-2508(94)90001-9. [DOI] [PubMed] [Google Scholar]
  13. Titgemeyer F., Walkenhorst J., Reizer J., Stuiver M. H., Cui X., Saier M. H., Jr Identification and characterization of phosphoenolpyruvate:fructose phosphotransferase systems in three Streptomyces species. Microbiology. 1995 Jan;141(Pt 1):51–58. doi: 10.1099/00221287-141-1-51. [DOI] [PubMed] [Google Scholar]
  14. Wu L. F., Tomich J. M., Saier M. H., Jr Structure and evolution of a multidomain multiphosphoryl transfer protein. Nucleotide sequence of the fruB(HI) gene in Rhodobacter capsulatus and comparisons with homologous genes from other organisms. J Mol Biol. 1990 Jun 20;213(4):687–703. doi: 10.1016/S0022-2836(05)80256-6. [DOI] [PubMed] [Google Scholar]
  15. Ye J. J., Neal J. W., Cui X., Reizer J., Saier M. H., Jr Regulation of the glucose:H+ symporter by metabolite-activated ATP-dependent phosphorylation of HPr in Lactobacillus brevis. J Bacteriol. 1994 Jun;176(12):3484–3492. doi: 10.1128/jb.176.12.3484-3492.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ye J. J., Reizer J., Cui X., Saier M. H., Jr ATP-dependent phosphorylation of serine-46 in the phosphocarrier protein HPr regulates lactose/H+ symport in Lactobacillus brevis. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3102–3106. doi: 10.1073/pnas.91.8.3102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ye J. J., Saier M. H., Jr Allosteric regulation of the glucose:H+ symporter of Lactobacillus brevis: cooperative binding of glucose and HPr(ser-P). J Bacteriol. 1995 Apr;177(7):1900–1902. doi: 10.1128/jb.177.7.1900-1902.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ye J. J., Saier M. H., Jr Cooperative binding of lactose and the phosphorylated phosphocarrier protein HPr(Ser-P) to the lactose/H+ symport permease of Lactobacillus brevis. Proc Natl Acad Sci U S A. 1995 Jan 17;92(2):417–421. doi: 10.1073/pnas.92.2.417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Zhu P. P., Reizer J., Reizer A., Peterkofsky A. Unique monocistronic operon (ptsH) in Mycoplasma capricolum encoding the phosphocarrier protein, HPr, of the phosphoenolpyruvate:sugar phosphotransferase system. Cloning, sequencing, and characterization of ptsH. J Biol Chem. 1993 Dec 15;268(35):26531–26540. [PubMed] [Google Scholar]
  20. de Crécy-Lagard V., Bouvet O. M., Lejeune P., Danchin A. Fructose catabolism in Xanthomonas campestris pv. campestris. Sequence of the PTS operon, characterization of the fructose-specific enzymes. J Biol Chem. 1991 Sep 25;266(27):18154–18161. [PubMed] [Google Scholar]

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