Abstract
1. Hamster stomach strips in vitro are useful test preparations for the assay of prostaglandins E and F in the nanogram concentration range. Three-minute cycles can be used for long periods without spontaneous contractions or significant base-line instability.
2. The mean percentage error and the precision index of six 4-point assays of prostaglandin E2 were 7·8 and 0·057, respectively.
3. The relative sensitivity to different prostaglandins was E2/E1∼1, E2/F1α∼65 and E2/A1∼400.
4. These preparations were relatively insensitive to 5-hydroxytryptamine (prostaglandin E2/5-HT∼20,000) and histamine (prostaglandin E2/histamine ∼200).
5. These results suggest that the hamster stomach preparation can be used to differentiate between 5-hydroxytryptamine and prostaglandins.
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Selected References
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