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. 1973 Dec;49(4):614–627. doi: 10.1111/j.1476-5381.1973.tb08537.x

Mechanism of efflux of noradrenaline from adrenergic nerves in rabbit atria

D M Paton
PMCID: PMC1776596  PMID: 4788035

Abstract

1. The mechanism of efflux of (-)-[3H]-noradrenaline was examined in rabbit atria, which were pretreated with reserpine and pargyline.

2. Between 40 and 100 min, efflux occurred predominantly from a single intraneuronal compartment.

3. Efflux was rapidy increased by (-)- and (+)-noradrenaline, tyramine and (±)-metaraminol, but not by (±)-isopropylnoradrenaline or (±)-normetanephrine. The increase in efflux produced by (-)-noradrenaline was inhibited by cocaine and desipramine but not by lidocaine.

4. Spontaneous effluxes, and those accelerated by (-)-noradrenaline, were temperature-sensitive.

5. Efflux was increased by ouabain, omission of K+, metabolic inhibition and lowering of the external Na+ concentration. These effects were significantly reduced by cocaine and desipramine but not by lidocaine.

6. These findings provide evidence that the efflux of [3H]-noradrenaline from adrenergic nerves occurs by a cocaine-sensitive, carrier-mediated process. The characteristics of the efflux process are compatible with, but not conclusive proof for, the Na+-gradient hypothesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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