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. 1974 Apr;50(4):569–574. doi: 10.1111/j.1476-5381.1974.tb08590.x

The accumulation of adenosine in rabbit intestinal muscle

Marilyn E Hulme, AH Weston
PMCID: PMC1776717  PMID: 4447859

Abstract

1 Strips of longitudinal muscle from rabbit intestine accumulated radioactivity when exposed to [3H]-adenosine.

2 Accumulation of radioactivity was not sodium-dependent or ouabain-sensitive, but was reduced by cooling, zero glucose plus bubbling with N2, 2,4,dinitrophenol, dipyridamole, hexobendine and lidoflazine.

3 After 7 min exposure to [3H]-adenosine, the tissue was found to contain radioactive adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP) and adenosine itself in the approximate ratio 13: 6: 4: 1.

4 In the presence of dipyridamole, hexobendine or lidoflazine (each 1 μM), the amounts of radioactive ATP, ADP, AMP and adenosine were reduced with the concentration of adenosine not significantly different from controls.

5 It is concluded that energy-dependent uptake of adenosine does not occur in the longitudinal muscle of rabbit intestine. Adenosine enters the tissue by a passive process and rapidly becomes phosphorylated giving rise to apparently high tissue: medium ratios.

6 The drugs dipyridamole, hexobendine and lidoflazine appear to reduce the accumulation of radioactivity by preventing the formation of adenosine phosphate derivatives.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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