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. 1974 Apr;50(4):543–551. doi: 10.1111/j.1476-5381.1974.tb08587.x

Inhibition by non-steroid anti-inflammatory agents of rabbit aorta contracting activity generated in blood by slow reacting substance C

SH Ferreira, BB Vargaftig
PMCID: PMC1776726  PMID: 4155653

Abstract

1 A crude and a partially purified preparation of slow reacting substance C (SRS-C) as well as arachidonic acid decreased resistance to perfusion of the dog hind paw. This effect was suppressed by treatment with non-steroid anti-inflammatory drugs.

2 Injections of SRS-C or of arachidonic acid induced marked and reproducible contractions of strips of rabbit aorta and a rat stomach which were bathed in blood from an anaesthetized dog. The effect on the rabbit aorta is attributed to formation of a rabbit aorta contracting substance (RCS). The contractions were suppressed when the dog was treated with a non-steroid anti-inflammatory drug.

3 Incubation of blood or of platelet-rich plasma with SRS-C or arachidonic acid resulted in the formation of similar materials. This formation was suppressed by anti-inflammatory drugs.

4 SRS-C, linoleic, linolenic, and arachidonic acids are suitable substrates for soybean lipoxidase for the generation of RCS.

5 It is suggested that RCS and prostaglandin are formed within platelets, when SRS-C or arachidonic acid are injected into animals or added in vitro. Non-steroid anti-inflammatory drugs suppress these effects, possibly by inhibiting prostaglandin synthetase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DESAI I. D., TAPPEL A. L. DAMAGE TO PROTEINS BY PEROXIDIZED LIPIDS. J Lipid Res. 1963 Apr;4:204–207. [PubMed] [Google Scholar]
  2. Ferreira S. H., Moncada S., Vane J. R. Indomethacin and aspirin abolish prostaglandin release from the spleen. Nat New Biol. 1971 Jun 23;231(25):237–239. doi: 10.1038/newbio231237a0. [DOI] [PubMed] [Google Scholar]
  3. Ferreira S. H. Prostaglandins, aspirin-like drugs and analgesia. Nat New Biol. 1972 Dec 13;240(102):200–203. doi: 10.1038/newbio240200a0. [DOI] [PubMed] [Google Scholar]
  4. Gilmore N., Vane J. R., Wyllie J. H. Prostaglandins released by the spleen. Nature. 1968 Jun 22;218(5147):1135–1140. doi: 10.1038/2181135a0. [DOI] [PubMed] [Google Scholar]
  5. Gryglewski R., Vane J. R. The generation from arachidonic acid of rabbit aorta contracting substance (RCS) by a microsomal enzyme preparation which also generates prostaglandins. Br J Pharmacol. 1972 Nov;46(3):449–457. doi: 10.1111/j.1476-5381.1972.tb08142.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hamberg M., Samuelsson B. Detection and isolation of an endoperoxide intermediate in prostaglandin biosynthesis. Proc Natl Acad Sci U S A. 1973 Mar;70(3):899–903. doi: 10.1073/pnas.70.3.899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hamberg M., Samuelsson B. On the specificity of the oxygenation of unsaturated fatty acids catalyzed by soybean lipoxidase. J Biol Chem. 1967 Nov 25;242(22):5329–5335. [PubMed] [Google Scholar]
  8. Higgs G. A., Youlten L. J. Proceedings: Prostaglandin production by rabbit peritoneal polymorphonuclear leukocytes in vitro. Br J Pharmacol. 1972 Feb;44(2):330P–330P. [PMC free article] [PubMed] [Google Scholar]
  9. Okuma M., Steiner M., Baldini G. Studies on lipid peroxides in platelets. II. Effect of aggregating agents and platelet antibody. J Lab Clin Med. 1971 May;77(5):728–742. [PubMed] [Google Scholar]
  10. Packham M. A., Mustard J. F. The effect of pyrazole compounds on thrombin-induced platelet aggregation. Proc Soc Exp Biol Med. 1969 Jan;130(1):72–75. doi: 10.3181/00379727-130-33490. [DOI] [PubMed] [Google Scholar]
  11. Piper P. J., Vane J. R. Release of additional factors in anaphylaxis and its antagonism by anti-inflammatory drugs. Nature. 1969 Jul 5;223(5201):29–35. doi: 10.1038/223029a0. [DOI] [PubMed] [Google Scholar]
  12. REGOLI D., VANE J. R. A SENSITIVE METHOD FOR THE ASSAY OF ANGIOTENSIN. Br J Pharmacol Chemother. 1964 Oct;23:351–359. doi: 10.1111/j.1476-5381.1964.tb01591.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Smith J. B., Willis A. L. Aspirin selectively inhibits prostaglandin production in human platelets. Nat New Biol. 1971 Jun 23;231(25):235–237. doi: 10.1038/newbio231235a0. [DOI] [PubMed] [Google Scholar]
  14. VANE J. R. A sensitive method for the assay of 5-hydroxytryptamine. Br J Pharmacol Chemother. 1957 Sep;12(3):344–349. doi: 10.1111/j.1476-5381.1957.tb00146.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. VANE J. R. THE USE OF ISOLATED ORGANS FOR DETECTING ACTIVE SUBSTANCES IN THE CIRCULATING BLOOD. Br J Pharmacol Chemother. 1964 Oct;23:360–373. doi: 10.1111/j.1476-5381.1964.tb01592.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. VOGT W. Pharmacologically active substances formed in egg yolk by cobra venom. J Physiol. 1957 Apr 3;136(1):131–147. doi: 10.1113/jphysiol.1957.sp005748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vane J. R. Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nat New Biol. 1971 Jun 23;231(25):232–235. doi: 10.1038/newbio231232a0. [DOI] [PubMed] [Google Scholar]
  18. Vargaftig B. B., Dao N. Release of vasoactive substances from guinea-pig lungs by slow-reacting substance c and arachidonic acid. Its blockade by nonsteroid anti-inflammatory agents. Pharmacology. 1971;6(2):99–108. doi: 10.1159/000136231. [DOI] [PubMed] [Google Scholar]
  19. Vargaftig B. B., De Miranda E. P., Lacoume B. Inhibition by non-steroidal anti-inflammatory agents of in vivo effects of "slow reacting substance C". Nature. 1969 May 31;222(5196):883–885. doi: 10.1038/222883a0. [DOI] [PubMed] [Google Scholar]
  20. Vargaftig B. B., Nguyen-dao-Hai Paradoxical inhibition of the effects of bradykinin by some sulfhydryl reagents. Experientia. 1972 Jan 15;28(1):59–62. doi: 10.1007/BF01928265. [DOI] [PubMed] [Google Scholar]
  21. Vargaftig B. B., Zirinis P. Platelet aggregation induced by arachidonic acid is accompanied by release of potential inflammatory mediators distinct from PGE2 and PGF2. Nat New Biol. 1973 Jul 25;244(134):114–116. doi: 10.1038/newbio244114a0. [DOI] [PubMed] [Google Scholar]

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