Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1996 Jan;178(2):542–545. doi: 10.1128/jb.178.2.542-545.1996

Salmonella typhimurium fimbrial phase variation and FimA expression.

S Clegg 1, L S Hancox 1, K S Yeh 1
PMCID: PMC177690  PMID: 8550478

Abstract

Bacteria in a nonfimbriate phase because of continuous aeration of liquid cultures produce FimA in amounts similar to those produced by fimbriate bacteria. However, relatively low FimA production was observed in nonfimbriate-phase cultures obtained by growth on solid media or by anaerobic incubation. Regardless of the fimbrial phase of Salmonella typhimurium, the fimA promoter region was always oriented in the direction that might allow fimA transcription.

Full Text

The Full Text of this article is available as a PDF (212.5 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abraham J. M., Freitag C. S., Clements J. R., Eisenstein B. I. An invertible element of DNA controls phase variation of type 1 fimbriae of Escherichia coli. Proc Natl Acad Sci U S A. 1985 Sep;82(17):5724–5727. doi: 10.1073/pnas.82.17.5724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blomfield I. C., McClain M. S., Princ J. A., Calie P. J., Eisenstein B. I. Type 1 fimbriation and fimE mutants of Escherichia coli K-12. J Bacteriol. 1991 Sep;173(17):5298–5307. doi: 10.1128/jb.173.17.5298-5307.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bullas L. R., Ryu J. I. Salmonella typhimurium LT2 strains which are r- m+ for all three chromosomally located systems of DNA restriction and modification. J Bacteriol. 1983 Oct;156(1):471–474. doi: 10.1128/jb.156.1.471-474.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DUGUID J. P. Fimbriae and adhesive properties in Klebsiella strains. J Gen Microbiol. 1959 Aug;21:271–286. doi: 10.1099/00221287-21-1-271. [DOI] [PubMed] [Google Scholar]
  5. Duguid J. P., Anderson E. S., Campbell I. Fimbriae and adhesive properties in Salmonellae. J Pathol Bacteriol. 1966 Jul;92(1):107–138. doi: 10.1002/path.1700920113. [DOI] [PubMed] [Google Scholar]
  6. Eisenstein B. I., Sweet D. S., Vaughn V., Friedman D. I. Integration host factor is required for the DNA inversion that controls phase variation in Escherichia coli. Proc Natl Acad Sci U S A. 1987 Sep;84(18):6506–6510. doi: 10.1073/pnas.84.18.6506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Freitag C. S., Abraham J. M., Clements J. R., Eisenstein B. I. Genetic analysis of the phase variation control of expression of type 1 fimbriae in Escherichia coli. J Bacteriol. 1985 May;162(2):668–675. doi: 10.1128/jb.162.2.668-675.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gerlach G. F., Allen B. L., Clegg S. Type 3 fimbriae among enterobacteria and the ability of spermidine to inhibit MR/K hemagglutination. Infect Immun. 1989 Jan;57(1):219–224. doi: 10.1128/iai.57.1.219-224.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hultgren S. J., Schwan W. R., Schaeffer A. J., Duncan J. L. Regulation of production of type 1 pili among urinary tract isolates of Escherichia coli. Infect Immun. 1986 Dec;54(3):613–620. doi: 10.1128/iai.54.3.613-620.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klemm P., Jørgensen B. J., van Die I., de Ree H., Bergmans H. The fim genes responsible for synthesis of type 1 fimbriae in Escherichia coli, cloning and genetic organization. Mol Gen Genet. 1985;199(3):410–414. doi: 10.1007/BF00330751. [DOI] [PubMed] [Google Scholar]
  11. Klemm P. Two regulatory fim genes, fimB and fimE, control the phase variation of type 1 fimbriae in Escherichia coli. EMBO J. 1986 Jun;5(6):1389–1393. doi: 10.1002/j.1460-2075.1986.tb04372.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McClain M. S., Blomfield I. C., Eberhardt K. J., Eisenstein B. I. Inversion-independent phase variation of type 1 fimbriae in Escherichia coli. J Bacteriol. 1993 Jul;175(14):4335–4344. doi: 10.1128/jb.175.14.4335-4344.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McClain M. S., Blomfield I. C., Eisenstein B. I. Roles of fimB and fimE in site-specific DNA inversion associated with phase variation of type 1 fimbriae in Escherichia coli. J Bacteriol. 1991 Sep;173(17):5308–5314. doi: 10.1128/jb.173.17.5308-5314.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Old D. C., Corneil I., Gibson L. F., Thomson A. D., Duguid J. P. Fimbriation, pellicle formation and the amount of growth of salmonellas in broth. J Gen Microbiol. 1968 Apr;51(1):1–16. doi: 10.1099/00221287-51-1-1. [DOI] [PubMed] [Google Scholar]
  15. Old D. C., Duguid J. P. Selective outgrowth of fimbriate bacteria in static liquid medium. J Bacteriol. 1970 Aug;103(2):447–456. doi: 10.1128/jb.103.2.447-456.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Old D. C., Duguid J. P. Transduction of fimbriation demonstrating common ancestry in FIRN strains of Salmonella typhimurium. J Gen Microbiol. 1979 Jun;112(2):251–259. doi: 10.1099/00221287-112-2-251. [DOI] [PubMed] [Google Scholar]
  17. Orndorff P. E., Falkow S. Organization and expression of genes responsible for type 1 piliation in Escherichia coli. J Bacteriol. 1984 Aug;159(2):736–744. doi: 10.1128/jb.159.2.736-744.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Purcell B. K., Pruckler J., Clegg S. Nucleotide sequences of the genes encoding type 1 fimbrial subunits of Klebsiella pneumoniae and Salmonella typhimurium. J Bacteriol. 1987 Dec;169(12):5831–5834. doi: 10.1128/jb.169.12.5831-5834.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Swenson D. L., Clegg S. Identification of ancillary fim genes affecting fimA expression in Salmonella typhimurium. J Bacteriol. 1992 Dec;174(23):7697–7704. doi: 10.1128/jb.174.23.7697-7704.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yeh K. S., Hancox L. S., Clegg S. Construction and characterization of a fimZ mutant of Salmonella typhimurium. J Bacteriol. 1995 Dec;177(23):6861–6865. doi: 10.1128/jb.177.23.6861-6865.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES