Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1996 Jan;178(2):550–553. doi: 10.1128/jb.178.2.550-553.1996

Multiple sigma factor genes in Brevibacterium lactofermentum: characterization of sigA and sigB.

J A Oguiza 1, A T Marcos 1, M Malumbres 1, J F Martín 1
PMCID: PMC177692  PMID: 8550480

Abstract

Four rpoD hybridizing signals have been identified in the chromosome of Brevibacterium lactofermentum. Two rpoD-like genes, sigA and sigB, have been cloned and sequenced, and they encode principal sigma factors of the RNA polymerase. The deduced amino acid sequences of SigA and SigB showed very high similarities to those of Mycobacterium smegmatis MysA and MysB proteins, respectively, and also to those of HrdB proteins from different Streptomyces species. SigA and SigB maintain the conserved motifs of sigma 70-like principal sigma factors. sigB is closely linked to the dtxR gene (encoding a repressor of iron-regulated promoters homologous to the diphtheria toxin repressor from Corynebacterium diphtheriae.

Full Text

The Full Text of this article is available as a PDF (282.5 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Araya T., Ishibashi N., Shimamura S., Tanaka K., Takahashi H. Genetic and molecular analysis of the rpoD gene from Lactococcus lactis. Biosci Biotechnol Biochem. 1993 Jan;57(1):88–92. doi: 10.1271/bbb.57.88. [DOI] [PubMed] [Google Scholar]
  2. Boyd J., Oza M. N., Murphy J. R. Molecular cloning and DNA sequence analysis of a diphtheria tox iron-dependent regulatory element (dtxR) from Corynebacterium diphtheriae. Proc Natl Acad Sci U S A. 1990 Aug;87(15):5968–5972. doi: 10.1073/pnas.87.15.5968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burgess R. R., Travers A. A., Dunn J. J., Bautz E. K. Factor stimulating transcription by RNA polymerase. Nature. 1969 Jan 4;221(5175):43–46. doi: 10.1038/221043a0. [DOI] [PubMed] [Google Scholar]
  4. Buttner M. J., Chater K. F., Bibb M. J. Cloning, disruption, and transcriptional analysis of three RNA polymerase sigma factor genes of Streptomyces coelicolor A3(2). J Bacteriol. 1990 Jun;172(6):3367–3378. doi: 10.1128/jb.172.6.3367-3378.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Deng Z. X., Kieser T., Hopwood D. A. Activity of a Streptomyces transcriptional terminator in Escherichia coli. Nucleic Acids Res. 1987 Mar 25;15(6):2665–2675. doi: 10.1093/nar/15.6.2665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Helmann J. D., Chamberlin M. J. Structure and function of bacterial sigma factors. Annu Rev Biochem. 1988;57:839–872. doi: 10.1146/annurev.bi.57.070188.004203. [DOI] [PubMed] [Google Scholar]
  7. Ingham C. J., Hunter I. S., Smith M. C. Rho-independent terminators without 3' poly-U tails from the early region of actinophage øC31. Nucleic Acids Res. 1995 Feb 11;23(3):370–376. doi: 10.1093/nar/23.3.370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kormanec J., Farkasovský M., Potúcková L. Four genes in Streptomyces aureofaciens containing a domain characteristic of principal sigma factors. Gene. 1992 Dec 1;122(1):63–70. doi: 10.1016/0378-1119(92)90032-k. [DOI] [PubMed] [Google Scholar]
  9. Lonetto M., Gribskov M., Gross C. A. The sigma 70 family: sequence conservation and evolutionary relationships. J Bacteriol. 1992 Jun;174(12):3843–3849. doi: 10.1128/jb.174.12.3843-3849.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Marcos A. T., Gutiérrez S., Díez B., Fernández F. J., Oguiza J. A., Martín J. F. Three genes hrdB, hrdD and hrdT of Streptomyces griseus IMRU 3570, encoding sigma factor-like proteins, are differentially expressed under specific nutritional conditions. Gene. 1995 Feb 3;153(1):41–48. doi: 10.1016/0378-1119(94)00759-l. [DOI] [PubMed] [Google Scholar]
  11. Mechold U., Steiner K., Vettermann S., Malke H. Genetic organization of the streptokinase region of the Streptococcus equisimilis H46A chromosome. Mol Gen Genet. 1993 Oct;241(1-2):129–140. doi: 10.1007/BF00280210. [DOI] [PubMed] [Google Scholar]
  12. Oguiza J. A., Tao X., Marcos A. T., Martín J. F., Murphy J. R. Molecular cloning, DNA sequence analysis, and characterization of the Corynebacterium diphtheriae dtxR homolog from Brevibacterium lactofermentum. J Bacteriol. 1995 Jan;177(2):465–467. doi: 10.1128/jb.177.2.465-467.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Plunkett G., 3rd, Burland V., Daniels D. L., Blattner F. R. Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes. Nucleic Acids Res. 1993 Jul 25;21(15):3391–3398. doi: 10.1093/nar/21.15.3391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Predich M., Doukhan L., Nair G., Smith I. Characterization of RNA polymerase and two sigma-factor genes from Mycobacterium smegmatis. Mol Microbiol. 1995 Jan;15(2):355–366. doi: 10.1111/j.1365-2958.1995.tb02249.x. [DOI] [PubMed] [Google Scholar]
  15. Sauer U., Treuner A., Buchholz M., Santangelo J. D., Dürre P. Sporulation and primary sigma factor homologous genes in Clostridium acetobutylicum. J Bacteriol. 1994 Nov;176(21):6572–6582. doi: 10.1128/jb.176.21.6572-6582.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tanaka K., Shiina T., Takahashi H. Multiple principal sigma factor homologs in eubacteria: identification of the "rpoD box". Science. 1988 Nov 18;242(4881):1040–1042. doi: 10.1126/science.3194753. [DOI] [PubMed] [Google Scholar]
  17. Tao X., Schiering N., Zeng H. Y., Ringe D., Murphy J. R. Iron, DtxR, and the regulation of diphtheria toxin expression. Mol Microbiol. 1994 Oct;14(2):191–197. doi: 10.1111/j.1365-2958.1994.tb01280.x. [DOI] [PubMed] [Google Scholar]
  18. Versalovic J., Koeuth T., Britton R., Geszvain K., Lupski J. R. Conservation and evolution of the rpsU-dnaG-rpoD macromolecular synthesis operon in bacteria. Mol Microbiol. 1993 Apr;8(2):343–355. doi: 10.1111/j.1365-2958.1993.tb01578.x. [DOI] [PubMed] [Google Scholar]
  19. Wang L. F., Doi R. H. Nucleotide sequence and organization of Bacillus subtilis RNA polymerase major sigma (sigma 43) operon. Nucleic Acids Res. 1986 May 27;14(10):4293–4307. doi: 10.1093/nar/14.10.4293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. van Wezel G. P., Buttner M. J., Vijgenboom E., Bosch L., Hopwood D. A., Kieser H. M. Mapping of genes involved in macromolecular synthesis on the chromosome of Streptomyces coelicolor A3(2). J Bacteriol. 1995 Jan;177(2):473–476. doi: 10.1128/jb.177.2.473-476.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES