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. 1996 Feb;178(3):917–921. doi: 10.1128/jb.178.3.917-921.1996

The repetitive element Rep MP 1 of Mycoplasma pneumoniae exists as a core element within a larger, variable repetitive mosaic.

M H Forsyth 1, S J Geary 1
PMCID: PMC177746  PMID: 8550534

Abstract

The repetitive element Rep MP 1 has been previously described as a 300-bp element present within the chromosome of Mycoplasma pneumoniae in at least 10 copies. Sequence found flanking Rep MP 1 in lambda clone 5B52 has been demonstrated to exist in multiple copies within the genome of M. pneumoniae. A HindIII M. pneumoniae fragment containing the lambda MP 5B52 insert and its flanking sequences was cloned into pBluescript. Sequence comparisons of this clone, designated pMPH 624-20, with nucleotide database entries showed that in addition to the 300-bp Rep MP 1 element, flanking sequence blocks were conserved within several other cloned sequences. These short repeated sequences, approximately 80, 56, and 71 nucleotides long, are termed sRep A, sRep B, and sRep C, respectively. These sRep regions were used as probes in the selection of cloned sequences containing these repetitive flanking regions. Comparison of these sequences demonstrated that the 300-bp Rep MP 1 elements is actually the core element within a larger, variable repetitive mosaic. The repetitive regions surrounding the core element are found in various combinations, arrangements, and distances from the core in a mosaic pattern. These newly identified portions of the mosaic do not exist independently of the core element. The core appears to be the only invariant portion of this repetitive mosaic.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. doi: 10.1016/S0022-2836(05)80360-2. [DOI] [PubMed] [Google Scholar]
  2. Bi X., Liu L. F. recA-independent and recA-dependent intramolecular plasmid recombination. Differential homology requirement and distance effect. J Mol Biol. 1994 Jan 14;235(2):414–423. doi: 10.1006/jmbi.1994.1002. [DOI] [PubMed] [Google Scholar]
  3. Colman S. D., Hu P. C., Bott K. F. Prevalence of novel repeat sequences in and around the P1 operon in the genome of Mycoplasma pneumoniae. Gene. 1990 Mar 1;87(1):91–96. doi: 10.1016/0378-1119(90)90498-g. [DOI] [PubMed] [Google Scholar]
  4. Correia F. F., Inouye S., Inouye M. A 26-base-pair repetitive sequence specific for Neisseria gonorrhoeae and Neisseria meningitidis genomic DNA. J Bacteriol. 1986 Sep;167(3):1009–1015. doi: 10.1128/jb.167.3.1009-1015.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Deng G., McIntosh M. A. An amplifiable DNA region from the Mycoplasma hyorhinis genome. J Bacteriol. 1994 Oct;176(19):5929–5937. doi: 10.1128/jb.176.19.5929-5937.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fetherston J. D., Schuetze P., Perry R. D. Loss of the pigmentation phenotype in Yersinia pestis is due to the spontaneous deletion of 102 kb of chromosomal DNA which is flanked by a repetitive element. Mol Microbiol. 1992 Sep;6(18):2693–2704. doi: 10.1111/j.1365-2958.1992.tb01446.x. [DOI] [PubMed] [Google Scholar]
  7. Haas R., Meyer T. F. The repertoire of silent pilus genes in Neisseria gonorrhoeae: evidence for gene conversion. Cell. 1986 Jan 17;44(1):107–115. doi: 10.1016/0092-8674(86)90489-7. [DOI] [PubMed] [Google Scholar]
  8. Halling S. M., Zehr E. S. Polymorphism in Brucella spp. due to highly repeated DNA. J Bacteriol. 1990 Dec;172(12):6637–6640. doi: 10.1128/jb.172.12.6637-6640.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hedén L. O., Lindahl G. Conserved and variable regions in protein Arp, the IgA receptor of Streptococcus pyogenes. J Gen Microbiol. 1993 Sep;139(9):2067–2074. doi: 10.1099/00221287-139-9-2067. [DOI] [PubMed] [Google Scholar]
  10. Iwaguchi S., Homma M., Chibana H., Tanaka K. Isolation and characterization of a repeated sequence (RPS1) of Candida albicans. J Gen Microbiol. 1992 Sep;138(9):1893–1900. doi: 10.1099/00221287-138-9-1893. [DOI] [PubMed] [Google Scholar]
  11. Lin R. J., Capage M., Hill C. W. A repetitive DNA sequence, rhs, responsible for duplications within the Escherichia coli K-12 chromosome. J Mol Biol. 1984 Jul 25;177(1):1–18. doi: 10.1016/0022-2836(84)90054-8. [DOI] [PubMed] [Google Scholar]
  12. Newbury S. F., Smith N. H., Robinson E. C., Hiles I. D., Higgins C. F. Stabilization of translationally active mRNA by prokaryotic REP sequences. Cell. 1987 Jan 30;48(2):297–310. doi: 10.1016/0092-8674(87)90433-8. [DOI] [PubMed] [Google Scholar]
  13. Nur I., LeBlanc D. J., Tully J. G. Short, interspersed, and repetitive DNA sequences in Spiroplasma species. Plasmid. 1987 Mar;17(2):110–116. doi: 10.1016/0147-619x(87)90017-5. [DOI] [PubMed] [Google Scholar]
  14. Proft T., Herrmann R. Identification and characterization of hitherto unknown Mycoplasma pneumoniae proteins. Mol Microbiol. 1994 Jul;13(2):337–348. doi: 10.1111/j.1365-2958.1994.tb00427.x. [DOI] [PubMed] [Google Scholar]
  15. Ruland K., Himmelreich R., Herrmann R. Sequence divergence in the ORF6 gene of Mycoplasma pneumonia. J Bacteriol. 1994 Sep;176(17):5202–5209. doi: 10.1128/jb.176.17.5202-5209.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ruland K., Wenzel R., Herrmann R. Analysis of three different repeated DNA elements present in the P1 operon of Mycoplasma pneumoniae: size, number and distribution on the genome. Nucleic Acids Res. 1990 Nov 11;18(21):6311–6317. doi: 10.1093/nar/18.21.6311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schaper U., Chapman J. S., Hu P. C. Preliminary indication of unusual codon usage in the DNA coding sequence of the attachment protein of Mycoplasma pneumoniae. Isr J Med Sci. 1987 May;23(5):361–367. [PubMed] [Google Scholar]
  19. Stern M. J., Prossnitz E., Ames G. F. Role of the intercistronic region in post-transcriptional control of gene expression in the histidine transport operon of Salmonella typhimurium: involvement of REP sequences. Mol Microbiol. 1988 Jan;2(1):141–152. doi: 10.1111/j.1365-2958.1988.tb00015.x. [DOI] [PubMed] [Google Scholar]
  20. Stringer S. L., Hong S. T., Giuntoli D., Stringer J. R. Repeated DNA in Pneumocystis carinii. J Clin Microbiol. 1991 Jun;29(6):1194–1201. doi: 10.1128/jcm.29.6.1194-1201.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Taylor M. A., Ferrell R. V., Wise K. S., McIntosh M. A. Reiterated DNA sequences defining genomic diversity within the species Mycoplasma hyorhinis. Mol Microbiol. 1988 Sep;2(5):665–672. doi: 10.1111/j.1365-2958.1988.tb00075.x. [DOI] [PubMed] [Google Scholar]
  22. Wenzel R., Herrmann R. Repetitive DNA sequences in Mycoplasma pneumoniae. Nucleic Acids Res. 1988 Sep 12;16(17):8337–8350. doi: 10.1093/nar/16.17.8337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Woese C. R. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. doi: 10.1128/mr.51.2.221-271.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Zuerner R. L., Bolin C. A. Repetitive sequence element cloned from Leptospira interrogans serovar hardjo type hardjo-bovis provides a sensitive diagnostic probe for bovine leptospirosis. J Clin Microbiol. 1988 Dec;26(12):2495–2500. doi: 10.1128/jcm.26.12.2495-2500.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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