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. 1996 Feb;178(3):922–926. doi: 10.1128/jb.178.3.922-926.1996

Transcription of the Anabaena sp. strain PCC 7120 ntcA gene: multiple transcripts and NtcA binding.

T S Ramasubramanian 1, T F Wei 1, A K Oldham 1, J W Golden 1
PMCID: PMC177747  PMID: 8550535

Abstract

The Anabaena sp. strain PCC 7120 ntcA gene showed multiple transcripts with different 5' ends. The relative abundance of transcripts varied in response to nitrogen availability. The ntcA product, NtcA, showed binding to the promoter region of its own gene. The binding site mapped to a region between the transcription start site used under nitrogen-replete conditions and the start sites used under nitrogen-limiting conditions, suggesting that NtcA regulates its own expression.

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Selected References

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  1. Bauer C. C., Haselkorn R. Vectors for determining the differential expression of genes in heterocysts and vegetative cells of Anabaena sp. strain PCC 7120. J Bacteriol. 1995 Jun;177(11):3332–3336. doi: 10.1128/jb.177.11.3332-3336.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buikema W. J., Haselkorn R. Characterization of a gene controlling heterocyst differentiation in the cyanobacterium Anabaena 7120. Genes Dev. 1991 Feb;5(2):321–330. doi: 10.1101/gad.5.2.321. [DOI] [PubMed] [Google Scholar]
  3. Carrasco C. D., Buettner J. A., Golden J. W. Programmed DNA rearrangement of a cyanobacterial hupL gene in heterocysts. Proc Natl Acad Sci U S A. 1995 Jan 31;92(3):791–795. doi: 10.1073/pnas.92.3.791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carrasco C. D., Ramaswamy K. S., Ramasubramanian T. S., Golden J. W. Anabaena xisF gene encodes a developmentally regulated site-specific recombinase. Genes Dev. 1994 Jan;8(1):74–83. doi: 10.1101/gad.8.1.74. [DOI] [PubMed] [Google Scholar]
  5. Chastain C. J., Brusca J. S., Ramasubramanian T. S., Wei T. F., Golden J. W. A sequence-specific DNA-binding factor (VF1) from Anabaena sp. strain PCC 7120 vegetative cells binds to three adjacent sites in the xisA upstream region. J Bacteriol. 1990 Sep;172(9):5044–5051. doi: 10.1128/jb.172.9.5044-5051.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cohen-Kupiec R., Zilberstein A., Gurevitz M. Characterization of cis elements that regulate the expression of glnA in Synechococcus sp. strain PCC 7942. J Bacteriol. 1995 Apr;177(8):2222–2226. doi: 10.1128/jb.177.8.2222-2226.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Frías J. E., Flores E., Herrero A. Requirement of the regulatory protein NtcA for the expression of nitrogen assimilation and heterocyst development genes in the cyanobacterium Anabaena sp. PCC 7120. Mol Microbiol. 1994 Nov;14(4):823–832. doi: 10.1111/j.1365-2958.1994.tb01318.x. [DOI] [PubMed] [Google Scholar]
  8. Gaston K., Bell A., Busby S., Fried M. A comparison of the DNA bending activities of the DNA binding proteins CRP and TFIID. Nucleic Acids Res. 1992 Jul 11;20(13):3391–3396. doi: 10.1093/nar/20.13.3391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Golden J. W., Robinson S. J., Haselkorn R. Rearrangement of nitrogen fixation genes during heterocyst differentiation in the cyanobacterium Anabaena. Nature. 1985 Apr 4;314(6010):419–423. doi: 10.1038/314419a0. [DOI] [PubMed] [Google Scholar]
  10. Golden J. W., Whorff L. L., Wiest D. R. Independent regulation of nifHDK operon transcription and DNA rearrangement during heterocyst differentiation in the cyanobacterium Anabaena sp. strain PCC 7120. J Bacteriol. 1991 Nov;173(22):7098–7105. doi: 10.1128/jb.173.22.7098-7105.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lammers P. J., Golden J. W., Haselkorn R. Identification and sequence of a gene required for a developmentally regulated DNA excision in Anabaena. Cell. 1986 Mar 28;44(6):905–911. doi: 10.1016/0092-8674(86)90013-9. [DOI] [PubMed] [Google Scholar]
  12. Luque I., Flores E., Herrero A. Molecular mechanism for the operation of nitrogen control in cyanobacteria. EMBO J. 1994 Jun 15;13(12):2862–2869. doi: 10.1002/j.1460-2075.1994.tb06580.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mohamed A., Jansson C. Influence of light on accumulation of photosynthesis-specific transcripts in the cyanobacterium Synechocystis 6803. Plant Mol Biol. 1989 Dec;13(6):693–700. doi: 10.1007/BF00016024. [DOI] [PubMed] [Google Scholar]
  14. Ramasubramanian T. S., Wei T. F., Golden J. W. Two Anabaena sp. strain PCC 7120 DNA-binding factors interact with vegetative cell- and heterocyst-specific genes. J Bacteriol. 1994 Mar;176(5):1214–1223. doi: 10.1128/jb.176.5.1214-1223.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Vega-Palas M. A., Flores E., Herrero A. NtcA, a global nitrogen regulator from the cyanobacterium Synechococcus that belongs to the Crp family of bacterial regulators. Mol Microbiol. 1992 Jul;6(13):1853–1859. doi: 10.1111/j.1365-2958.1992.tb01357.x. [DOI] [PubMed] [Google Scholar]
  16. Vega-Palas M. A., Madueño F., Herrero A., Flores E. Identification and cloning of a regulatory gene for nitrogen assimilation in the cyanobacterium Synechococcus sp. strain PCC 7942. J Bacteriol. 1990 Feb;172(2):643–647. doi: 10.1128/jb.172.2.643-647.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wei T. F., Ramasubramanian T. S., Golden J. W. Anabaena sp. strain PCC 7120 ntcA gene required for growth on nitrate and heterocyst development. J Bacteriol. 1994 Aug;176(15):4473–4482. doi: 10.1128/jb.176.15.4473-4482.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wei T. F., Ramasubramanian T. S., Pu F., Golden J. W. Anabaena sp. strain PCC 7120 bifA gene encoding a sequence-specific DNA-binding protein cloned by in vivo transcriptional interference selection. J Bacteriol. 1993 Jul;175(13):4025–4035. doi: 10.1128/jb.175.13.4025-4035.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

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