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. 1996 Feb;178(3):940–942. doi: 10.1128/jb.178.3.940-942.1996

Isolation and characterization of a mutation that alters the substrate specificity of the Escherichia coli glucose permease.

G S Begley 1, K A Warner 1, J C Arents 1, P W Postma 1, G R Jacobson 1
PMCID: PMC177751  PMID: 8550539

Abstract

We isolated 10 mannitol-positive mutants from a mannitol-negative Escherichia coli strain. These mutations mapped within ptsG, encoding the glucose permease (EIIGlc), and resulted in a G-320-to-V substitution that allows EIIGlc to transport mannitol. Gly-320 lies within a putative transmembrane helix of EIIGlc that may be involved in substrate recognition.

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Selected References

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