Abstract
A genomic library from Streptomyces tendae raised in shuttle cosmid vector pKC505 was screened with a previously isolated 8-kb DNA fragment containing the orfP1 gene, which is involved in nikkomycin biosynthesis. The entire set of structural genes for nikkomycin synthesis was heterologously expressed in S. lividans TK23 by introducing recombinant cosmids p24/32 and p9/43-2, carrying inserts of about 31 and 27 kb, respectively, overlapping by 15 kb. S. lividans transformants synthesized nikkomycins X, Z, I, and J, which were identified by high-pressure liquid chromatography analyses of culture filtrates.
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- Bormann C., Huhn W., Zähner H., Rathmann R., Hahn H., König W. A. Metabolic products of microorganisms. 228. New nikkomycins produced by mutants of Streptomyces tendae. J Antibiot (Tokyo) 1985 Jan;38(1):9–16. doi: 10.7164/antibiotics.38.9. [DOI] [PubMed] [Google Scholar]
- Bormann C., Mattern S., Schrempf H., Fiedler H. P., Zähner H. Isolation of Streptomyces tendae mutants with an altered nikkomycin spectrum. J Antibiot (Tokyo) 1989 Jun;42(6):913–918. doi: 10.7164/antibiotics.42.913. [DOI] [PubMed] [Google Scholar]
- Brillinger G. U. Metabolic products of microorganisms. 181. Chitin synthase from fungi, a test model for substances with insecticidal properties. Arch Microbiol. 1979 Apr;121(1):71–74. doi: 10.1007/BF00409207. [DOI] [PubMed] [Google Scholar]
- Dähn U., Hagenmaier H., Höhne H., König W. A., Wolf G., Zähner H. Stoffwechselprodukte von mikroorganismen. 154. Mitteilung. Nikkomycin, ein neuer hemmstoff der chitinsynthese bei pilzen. Arch Microbiol. 1976 Mar 19;107(2):143–160. doi: 10.1007/BF00446834. [DOI] [PubMed] [Google Scholar]
- Fiedler H. P. Screening for new microbial products by high-performance liquid chromatography using a photodiode array detector. J Chromatogr. 1984 Dec 21;316:487–494. doi: 10.1016/s0021-9673(00)96176-4. [DOI] [PubMed] [Google Scholar]
- Martín M. F., Liras P. Organization and expression of genes involved in the biosynthesis of antibiotics and other secondary metabolites. Annu Rev Microbiol. 1989;43:173–206. doi: 10.1146/annurev.mi.43.100189.001133. [DOI] [PubMed] [Google Scholar]
- Möhrle V., Roos U., Bormann C. Identification of cellular proteins involved in nikkomycin production in Streptomyces tendae Tü901. Mol Microbiol. 1995 Feb;15(3):561–571. doi: 10.1111/j.1365-2958.1995.tb02269.x. [DOI] [PubMed] [Google Scholar]
- Richardson M. A., Kuhstoss S., Solenberg P., Schaus N. A., Rao R. N. A new shuttle cosmid vector, pKC505, for streptomycetes: its use in the cloning of three different spiramycin-resistance genes from a Streptomyces ambofaciens library. Gene. 1987;61(3):231–241. doi: 10.1016/0378-1119(87)90187-9. [DOI] [PubMed] [Google Scholar]