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. 1974 Aug;51(4):521–531. doi: 10.1111/j.1476-5381.1974.tb09670.x

The facilitatory actions of 5-hydroxytryptamine and bradykinin in the superior cervical ganglion of the rabbit

DI Wallis, B Woodward
PMCID: PMC1778055  PMID: 4375529

Abstract

1 The effects of 5-hydroxytryptamine (5-HT) on ganglionic transmission and on intrinsic modulation of transmission have been re-examined and compared with the effects of bradykinin by means of electrophysiological techniques.

2 Early facilitation, which is maximal 40-75 ms after a conditioning stimulus, was considerably enhanced by 5-HT. This enhancement was concentration-dependent, the threshold concentration lying between 0.1 and 1 μM. With concentrations of 5-HT 10 μM or greater, there was some depression of the Sa response to the conditioning stimulus.

3 5-HT reduced or abolished the inhibition of a test response induced by a conditioning response 100-300 ms earlier. Facilitation was observed at these intervals at concentrations of 5-HT of 25 μM or greater.

4 Late facilitation, which is maximal 700-2000 ms after a conditioning stimulus, was increased by 5-HT, but the effect was not as great as on early facilitation and was not always seen with a concentration of 1 μM.

5 Bradykinin reduced early facilitation but increased the amplitude of the transmitted action potential in response to a single stimulus. The threshold concentration producing these effects was between 1 and 2 μM.

6 5-HT produced a rapid depolarization of the ganglion cell membrane which was followed by an after-hyperpolarization.

7 Bradykinin either produced no measurable change in ganglion cell resting potential or only very small, transient depolarizations.

8 The depression of transmission, enhancement of intrinsic facilitation and the depolarization of the ganglion cell membrane induced by 5-HT may indicate more than one mode of action of this amine at the ganglionic synapse.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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