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. 1999 Aug 3;96(16):9368–9373. doi: 10.1073/pnas.96.16.9368

Figure 1.

Figure 1

hrp-dependent secretion of AvrBs3. Bacteria were incubated for 4 hr in minimal medium MA at pH 5.4 with BSA (50 μg/ml). Total protein extracts (T, lanes 1 and 2) and filtered supernatants (SN, lanes 3 and 4) were precipitated with TCA and concentrated 10- and 50-fold, respectively. Five-microliter protein samples of strains 82*(pDGUS) (lanes 1 and 3) and 82*ΔhrcV(pDGUS) (lanes 2 and 4) were separated by SDS/PAGE (8% polyacrylamide) and analyzed by immunoblotting by using polyclonal antibodies directed against AvrBs3 (upper blot) and HrcN (lower blot). The molecular mass (kDa) of the proteins is indicated. For β-glucuronidase activities, see text.