Abstract
Regulation of the ilv-leu operon probably involves interaction of a tR NA(GAG) with leader region mRNA. Conversion of a CUC (Leu) triplet located within the leader region to UUC (Phe), CGC (Arg), or UAC (Tyr) converted reporter gene expression to control by corresponding amino acids. Conversion of the CUC triplet to CUU (Leu) decreased expression and disrupted regulation. The results suggested that other tRNAs can substitute for tRNA(Leu) but that interactions in addition to pairing of the anticodon with the CUC triplet are important for proper control.
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- Gagnon Y., Breton R., Putzer H., Pelchat M., Grunberg-Manago M., Lapointe J. Clustering and co-transcription of the Bacillus subtilis genes encoding the aminoacyl-tRNA synthetases specific for glutamate and for cysteine and the first enzyme for cysteine biosynthesis. J Biol Chem. 1994 Mar 11;269(10):7473–7482. [PubMed] [Google Scholar]
- Garrity D. B., Zahler S. A. Mutations in the gene for a tRNA that functions as a regulator of a transcriptional attenuator in Bacillus subtilis. Genetics. 1994 Jul;137(3):627–636. doi: 10.1093/genetics/137.3.627. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Garrity D. B., Zahler S. A. The Bacillus subtilis ochre suppressor sup-3 is located in an operon of seven tRNA genes. J Bacteriol. 1993 Oct;175(20):6512–6517. doi: 10.1128/jb.175.20.6512-6517.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gauss D. H., Sprinzl M. Compilation of tRNA sequences. Nucleic Acids Res. 1983 Jan 11;11(1):r1–53. [PMC free article] [PubMed] [Google Scholar]
- Grandoni J. A., Fulmer S. B., Brizzio V., Zahler S. A., Calvo J. M. Regions of the Bacillus subtilis ilv-leu operon involved in regulation by leucine. J Bacteriol. 1993 Dec;175(23):7581–7593. doi: 10.1128/jb.175.23.7581-7593.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grandoni J. A., Zahler S. A., Calvo J. M. Transcriptional regulation of the ilv-leu operon of Bacillus subtilis. J Bacteriol. 1992 May;174(10):3212–3219. doi: 10.1128/jb.174.10.3212-3219.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grundy F. J., Henkin T. M. Conservation of a transcription antitermination mechanism in aminoacyl-tRNA synthetase and amino acid biosynthesis genes in gram-positive bacteria. J Mol Biol. 1994 Jan 14;235(2):798–804. doi: 10.1006/jmbi.1994.1038. [DOI] [PubMed] [Google Scholar]
- Grundy F. J., Henkin T. M. tRNA as a positive regulator of transcription antitermination in B. subtilis. Cell. 1993 Aug 13;74(3):475–482. doi: 10.1016/0092-8674(93)80049-k. [DOI] [PubMed] [Google Scholar]
- Grundy F. J., Rollins S. M., Henkin T. M. Interaction between the acceptor end of tRNA and the T box stimulates antitermination in the Bacillus subtilis tyrS gene: a new role for the discriminator base. J Bacteriol. 1994 Aug;176(15):4518–4526. doi: 10.1128/jb.176.15.4518-4526.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Henkin T. M. tRNA-directed transcription antitermination. Mol Microbiol. 1994 Aug;13(3):381–387. doi: 10.1111/j.1365-2958.1994.tb00432.x. [DOI] [PubMed] [Google Scholar]
- Kunkel T. A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci U S A. 1985 Jan;82(2):488–492. doi: 10.1073/pnas.82.2.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Putzer H., Laalami S., Brakhage A. A., Condon C., Grunberg-Manago M. Aminoacyl-tRNA synthetase gene regulation in Bacillus subtilis: induction, repression and growth-rate regulation. Mol Microbiol. 1995 May;16(4):709–718. doi: 10.1111/j.1365-2958.1995.tb02432.x. [DOI] [PubMed] [Google Scholar]
- Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wawrousek E. F., Narasimhan N., Hansen J. N. Two large clusters with thirty-seven transfer RNA genes adjacent to ribosomal RNA gene sets in Bacillus subtilis. Sequence and organization of trrnD and trrnE gene clusters. J Biol Chem. 1984 Mar 25;259(6):3694–3702. [PubMed] [Google Scholar]