Abstract
Genes encoding the ammonia-dependent asparagine synthetase (asnA) and asparaginyl-tRNA synthetase (asnS) have been cloned from Lactobacillus bulgaricus ATCC 11842. The nucleotide sequence suggests that asnA and asnS are organized as one operon and regulated by the tRNA-directed transcription antitermination mechanism (T. M. Henkin, Mol. Microbiol. 13:381-387, 1994).
Full Text
The Full Text of this article is available as a PDF (186.9 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Andrulis I. L., Chen J., Ray P. N. Isolation of human cDNAs for asparagine synthetase and expression in Jensen rat sarcoma cells. Mol Cell Biol. 1987 Jul;7(7):2435–2443. doi: 10.1128/mcb.7.7.2435. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Andrulis I. L., Shotwell M., Evans-Blackler S., Zalkin H., Siminovitch L., Ray P. N. Fine structure analysis of the Chinese hamster AS gene encoding asparagine synthetase. Gene. 1989 Aug 1;80(1):75–85. doi: 10.1016/0378-1119(89)90252-7. [DOI] [PubMed] [Google Scholar]
- Cedar H., Schwartz J. H. The asparagine synthetase of Escherhic coli. I. Biosynthetic role of the enzyme, purification, and characterization of the reaction products. J Biol Chem. 1969 Aug 10;244(15):4112–4121. [PubMed] [Google Scholar]
- Felton J., Michaelis S., Wright A. Mutations in two unlinked genes are required to produce asparagine auxotrophy in Escherichia coli. J Bacteriol. 1980 Apr;142(1):221–228. doi: 10.1128/jb.142.1.221-228.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gagnon Y., Breton R., Putzer H., Pelchat M., Grunberg-Manago M., Lapointe J. Clustering and co-transcription of the Bacillus subtilis genes encoding the aminoacyl-tRNA synthetases specific for glutamate and for cysteine and the first enzyme for cysteine biosynthesis. J Biol Chem. 1994 Mar 11;269(10):7473–7482. [PubMed] [Google Scholar]
- Grundy F. J., Henkin T. M. tRNA as a positive regulator of transcription antitermination in B. subtilis. Cell. 1993 Aug 13;74(3):475–482. doi: 10.1016/0092-8674(93)80049-k. [DOI] [PubMed] [Google Scholar]
- Henkin T. M. tRNA-directed transcription antitermination. Mol Microbiol. 1994 Aug;13(3):381–387. doi: 10.1111/j.1365-2958.1994.tb00432.x. [DOI] [PubMed] [Google Scholar]
- Hinchman S. K., Henikoff S., Schuster S. M. A relationship between asparagine synthetase A and aspartyl tRNA synthetase. J Biol Chem. 1992 Jan 5;267(1):144–149. [PubMed] [Google Scholar]
- Kölling R., Lother H. AsnC: an autogenously regulated activator of asparagine synthetase A transcription in Escherichia coli. J Bacteriol. 1985 Oct;164(1):310–315. doi: 10.1128/jb.164.1.310-315.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lam H. M., Peng S. S., Coruzzi G. M. Metabolic regulation of the gene encoding glutamine-dependent asparagine synthetase in Arabidopsis thaliana. Plant Physiol. 1994 Dec;106(4):1347–1357. doi: 10.1104/pp.106.4.1347. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leong-Morgenthaler P., Zwahlen M. C., Hottinger H. Lactose metabolism in Lactobacillus bulgaricus: analysis of the primary structure and expression of the genes involved. J Bacteriol. 1991 Mar;173(6):1951–1957. doi: 10.1128/jb.173.6.1951-1957.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nakamura M., Yamada M., Hirota Y., Sugimoto K., Oka A., Takanami M. Nucleotide sequence of the asnA gene coding for asparagine synthetase of E. coli K-12. Nucleic Acids Res. 1981 Sep 25;9(18):4669–4676. doi: 10.1093/nar/9.18.4669. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scofield M. A., Lewis W. S., Schuster S. M. Nucleotide sequence of Escherichia coli asnB and deduced amino acid sequence of asparagine synthetase B. J Biol Chem. 1990 Aug 5;265(22):12895–12902. [PubMed] [Google Scholar]
- Tabor S., Richardson C. C. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074–1078. doi: 10.1073/pnas.82.4.1074. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Wind N., de Jong M., Meijer M., Stuitje A. R. Site-directed mutagenesis of the Escherichia coli chromosome near oriC: identification and characterization of asnC, a regulatory element in E. coli asparagine metabolism. Nucleic Acids Res. 1985 Dec 20;13(24):8797–8811. doi: 10.1093/nar/13.24.8797. [DOI] [PMC free article] [PubMed] [Google Scholar]