FIGURE 2.

NMR spectroscopy of P4 in the absence of Mg²⁺. (A) Expanded region of the nonexchangable NOESY of P4 showing the NOE walk for the A3–C13 strand. (B) Detection of hydrogen bond alignments using the J_NN-COSY experiment (Dingley and Grzesiek 1998; Pervushin et al. 1998). The measured ^2hJ_NN coupling constants (in hertz) are shown adjacent to each cross peak (Dingley et al. 1999). (C) One bond C–H RDCs measured in P4 as a function of residue.