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. 2007 Feb;13(2):267–280. doi: 10.1261/rna.243607

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FIGURE 6. — Analyses of interactions between the hexanucleotide element and multiple internal elements in vivo. (A,B) Accumulation of minus-strand RNA in protoplasts inoculated with full-length transcripts from wild-type, single element mutants, or multiple element mutants. Control reactions in lanes 1 and 2 are as described in the Figure 5 legend (panel C). Lanes 3 and 5 in panel A contain protected minus-strand RNA fragments from protoplasts inoculated with wild-type or p32 (replicase defective control), respectively. Products from protoplasts inoculated with single element mutants, Pol and CP8, are in lanes 4 and 6, respectively. Products from the TB8CP8 double element mutant and the PolTB8CP8 triple element mutant are in lanes 7 and 8. Similarly, panel B includes products from protoplasts inoculated with the single element mutant, TB₂, (lane 5), double element mutant TB8CP8 (lane 6), and additional multiple element mutants, PolTB8CP8, TB8TB₂CP8, and PolTB9TB₂CP9 (lanes 7–9). (C) Quantitative representation of data in panels A and B.