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. 1996 Jun;178(12):3658–3660. doi: 10.1128/jb.178.12.3658-3660.1996

Characterization of the major citrate synthase of Bacillus subtilis.

S Jin 1, A L Sonenshein 1
PMCID: PMC178141  PMID: 8655569

Abstract

The major citrate synthase of Bacillus subtilis (CS-II) was purified to near homogeneity and shown to correspond to the product of the citZ gene. Accumulation of CS-II during exponential growth and stationary phases paralleled expression of the citZ gene. The physical and kinetic properties of CS-II were similar to those of citrate synthase enzymes from Bacillus megaterium and from eukaryotic cells but differed from those of citrate synthases from many gram-negative bacteria.

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Selected References

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  1. Alter G. M., Casazza J. P., Zhi W., Nemeth P., Srere P. A., Evans C. T. Mutation of essential catalytic residues in pig citrate synthase. Biochemistry. 1990 Aug 21;29(33):7557–7563. doi: 10.1021/bi00485a003. [DOI] [PubMed] [Google Scholar]
  2. Bryan J. K. Molecular weights of protein multimers from polyacrylamide gel electrophoresis. Anal Biochem. 1977 Apr;78(2):513–519. doi: 10.1016/0003-2697(77)90111-7. [DOI] [PubMed] [Google Scholar]
  3. Dingman D. W., Rosenkrantz M. S., Sonenshein A. L. Relationship between aconitase gene expression and sporulation in Bacillus subtilis. J Bacteriol. 1987 Jul;169(7):3068–3075. doi: 10.1128/jb.169.7.3068-3075.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Flechtner V. R., Hanson R. S. Coarse and fine control of citrate synthase from Bacillus subtilis. Biochim Biophys Acta. 1969 Jul 30;184(2):252–262. doi: 10.1016/0304-4165(69)90027-0. [DOI] [PubMed] [Google Scholar]
  5. Fortnagel P., Freese E. Analysis of sporulation mutants. II. Mutants blocked in the citric acid cycle. J Bacteriol. 1968 Apr;95(4):1431–1438. doi: 10.1128/jb.95.4.1431-1438.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jin S., Sonenshein A. L. Identification of two distinct Bacillus subtilis citrate synthase genes. J Bacteriol. 1994 Aug;176(15):4669–4679. doi: 10.1128/jb.176.15.4669-4679.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jin S., Sonenshein A. L. Transcriptional regulation of Bacillus subtilis citrate synthase genes. J Bacteriol. 1994 Aug;176(15):4680–4690. doi: 10.1128/jb.176.15.4680-4690.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Johnson D. E., Hanson R. S. Bacterial citrate synthases: purification, molecular weight and kinetic mechanism. Biochim Biophys Acta. 1974 Jun 18;350(2):336–353. doi: 10.1016/0005-2744(74)90508-7. [DOI] [PubMed] [Google Scholar]
  9. Kubicek C. P., Röhr M. Regulation of citrate synthase from the citric acid-accumulating fungus, Aspergillus niger. Biochim Biophys Acta. 1980 Oct;615(2):449–457. [PubMed] [Google Scholar]
  10. Löhlein-Werhahn G., Goepfert P., Eggerer H. Purification and properties of an archaebacterial enzyme: citrate synthase from Sulfolobus solfataricus. Biol Chem Hoppe Seyler. 1988 Feb;369(2):109–113. doi: 10.1515/bchm3.1988.369.1.109. [DOI] [PubMed] [Google Scholar]
  11. Matlib M. A., Boesman-Finkelstein M., Srere P. A. The kinetics of rat liver citrate synthase in situ. Arch Biochem Biophys. 1978 Dec;191(2):426–430. doi: 10.1016/0003-9861(78)90380-6. [DOI] [PubMed] [Google Scholar]
  12. Morse D., Duckworth H. W. A comparison of the citrate synthases of Escherichia coli and Acinetobacter anitratum. Can J Biochem. 1980 Sep;58(9):696–706. doi: 10.1139/o80-098. [DOI] [PubMed] [Google Scholar]
  13. Patton A. J., Hough D. W., Towner P., Danson M. J. Does Escherichia coli possess a second citrate synthase gene? Eur J Biochem. 1993 May 15;214(1):75–81. doi: 10.1111/j.1432-1033.1993.tb17898.x. [DOI] [PubMed] [Google Scholar]
  14. Pereira D. S., Donald L. J., Hosfield D. J., Duckworth H. W. Active site mutants of Escherichia coli citrate synthase. Effects of mutations on catalytic and allosteric properties. J Biol Chem. 1994 Jan 7;269(1):412–417. [PubMed] [Google Scholar]
  15. Rault-Leonardon M., Atkinson M. A., Slaughter C. A., Moomaw C. R., Srere P. A. Azotobacter vinelandii citrate synthase. Biochemistry. 1995 Jan 10;34(1):257–263. doi: 10.1021/bi00001a031. [DOI] [PubMed] [Google Scholar]
  16. Robinson M. S., Danson M. J., Weitzman P. D. Citrate synthase from a Gram-positive bacterium. Purification and characterization of the Bacillus megaterium enzyme. Biochem J. 1983 Jul 1;213(1):53–59. doi: 10.1042/bj2130053. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schendel F. J., August P. R., Anderson C. R., Hanson R. S., Flickinger M. C. Cloning and nucleotide sequence of the gene coding for citrate synthase from a thermotolerant Bacillus sp. Appl Environ Microbiol. 1992 Jan;58(1):335–345. doi: 10.1128/aem.58.1.335-345.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Swissa M., Benziman M. Factors affecting the activity of citrate synthase of Acetobacter xylinum and its possible regulatory role. Biochem J. 1976 Feb 1;153(2):173–179. doi: 10.1042/bj1530173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Weitzman P. D., Danson M. J. Citrate synthase. Curr Top Cell Regul. 1976;10:161–204. doi: 10.1016/b978-0-12-152810-2.50011-5. [DOI] [PubMed] [Google Scholar]
  20. Weitzman P. D. Unity and diversity in some bacterial citric acid-cycle enzymes. Adv Microb Physiol. 1981;22:185–244. doi: 10.1016/s0065-2911(08)60328-8. [DOI] [PubMed] [Google Scholar]

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