Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1996 Jun;178(12):3661–3663. doi: 10.1128/jb.178.12.3661-3663.1996

Genetic analysis of plasmid determinants for microcin J25 production and immunity.

J O Solbiati 1, M Ciaccio 1, R N Farías 1, R A Salomón 1
PMCID: PMC178142  PMID: 8655570

Abstract

Microcin J25 (MccJ25) is a small peptide antibiotic produced by an Escherichia coli strain isolated from human feces. The genetic determinants for MccJ25 synthesis and immunity have been cloned from the low-copy-number wild-type plasmid pTUC1OO into the compatible vectors pBR322 and pACYC184. Physical and phenotypical analysis of insertion mutations and complementation tests defined three contiguous genes involved in MccJ25 production which span a region of about 2.2 kb. Immunity to the antibiotic is provided by an additional gene adjacent to the production region.

Full Text

The Full Text of this article is available as a PDF (202.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arakawa S., Kuramitsu H. K. Cloning and sequence analysis of a chymotrypsinlike protease from Treponema denticola. Infect Immun. 1994 Aug;62(8):3424–3433. doi: 10.1128/iai.62.8.3424-3433.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Asensio C., Pérez-Díaz J. C. A new family of low molecular weight antibiotics from enterobacteria. Biochem Biophys Res Commun. 1976 Mar 8;69(1):7–14. doi: 10.1016/s0006-291x(76)80264-1. [DOI] [PubMed] [Google Scholar]
  3. Baehni P. C., Song M., McCulloch C. A., Ellen R. P. Treponema denticola induces actin rearrangement and detachment of human gingival fibroblasts. Infect Immun. 1992 Aug;60(8):3360–3368. doi: 10.1128/iai.60.8.3360-3368.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berg D. E., Weiss A., Crossland L. Polarity of Tn5 insertion mutations in Escherichia coli. J Bacteriol. 1980 May;142(2):439–446. doi: 10.1128/jb.142.2.439-446.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chan E. C., Qiu Y. S., Siboo R., Noble P. Evidence for two distinct locomotory phenotypes of Treponema denticola ATCC 35405. Oral Microbiol Immunol. 1995 Apr;10(2):122–124. doi: 10.1111/j.1399-302x.1995.tb00131.x. [DOI] [PubMed] [Google Scholar]
  6. Charon N. W., Greenberg E. P., Koopman M. B., Limberger R. J. Spirochete chemotaxis, motility, and the structure of the spirochetal periplasmic flagella. Res Microbiol. 1992 Jul-Aug;143(6):597–603. doi: 10.1016/0923-2508(92)90117-7. [DOI] [PubMed] [Google Scholar]
  7. Dahle U. R., Tronstad L., Olsen I. Spirochaetes in oral infections. Endod Dent Traumatol. 1993 Jun;9(3):87–94. doi: 10.1111/j.1600-9657.1993.tb00257.x. [DOI] [PubMed] [Google Scholar]
  8. Fletcher H. M., Schenkein H. A., Morgan R. M., Bailey K. A., Berry C. R., Macrina F. L. Virulence of a Porphyromonas gingivalis W83 mutant defective in the prtH gene. Infect Immun. 1995 Apr;63(4):1521–1528. doi: 10.1128/iai.63.4.1521-1528.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gaggero C., Moreno F., Laviña M. Genetic analysis of microcin H47 antibiotic system. J Bacteriol. 1993 Sep;175(17):5420–5427. doi: 10.1128/jb.175.17.5420-5427.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gilson L., Mahanty H. K., Kolter R. Four plasmid genes are required for colicin V synthesis, export, and immunity. J Bacteriol. 1987 Jun;169(6):2466–2470. doi: 10.1128/jb.169.6.2466-2470.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jack R. W., Tagg J. R., Ray B. Bacteriocins of gram-positive bacteria. Microbiol Rev. 1995 Jun;59(2):171–200. doi: 10.1128/mr.59.2.171-200.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Laviña M., Gaggero C., Moreno F. Microcin H47, a chromosome-encoded microcin antibiotic of Escherichia coli. J Bacteriol. 1990 Nov;172(11):6585–6588. doi: 10.1128/jb.172.11.6585-6588.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Laviña M., Pugsley A. P., Moreno F. Identification, mapping, cloning and characterization of a gene (sbmA) required for microcin B17 action on Escherichia coli K12. J Gen Microbiol. 1986 Jun;132(6):1685–1693. doi: 10.1099/00221287-132-6-1685. [DOI] [PubMed] [Google Scholar]
  14. Limberger R. J., Charon N. W. Treponema phagedenis has at least two proteins residing together on its periplasmic flagella. J Bacteriol. 1986 Apr;166(1):105–112. doi: 10.1128/jb.166.1.105-112.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Limberger R. J., Slivienski L. L., Samsonoff W. A. Genetic and biochemical analysis of the flagellar hook of Treponema phagedenis. J Bacteriol. 1994 Jun;176(12):3631–3637. doi: 10.1128/jb.176.12.3631-3637.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Loesche W. J. Bacterial mediators in periodontal disease. Clin Infect Dis. 1993 Jun;16 (Suppl 4):S203–S210. doi: 10.1093/clinids/16.supplement_4.s203. [DOI] [PubMed] [Google Scholar]
  17. Loesche W. J. The role of spirochetes in periodontal disease. Adv Dent Res. 1988 Nov;2(2):275–283. doi: 10.1177/08959374880020021201. [DOI] [PubMed] [Google Scholar]
  18. Macnab R. M. Genetics and biogenesis of bacterial flagella. Annu Rev Genet. 1992;26:131–158. doi: 10.1146/annurev.ge.26.120192.001023. [DOI] [PubMed] [Google Scholar]
  19. Novoa M. A., Díaz-Guerra L., San Millán J. L., Moreno F. Cloning and mapping of the genetic determinants for microcin C7 production and immunity. J Bacteriol. 1986 Dec;168(3):1384–1391. doi: 10.1128/jb.168.3.1384-1391.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Riviere G. R., Elliot K. S., Adams D. F., Simonson L. G., Forgas L. B., Nilius A. M., Lukehart S. A. Relative proportions of pathogen-related oral spirochetes (PROS) and Treponema denticola in supragingival and subgingival plaque from patients with periodontitis. J Periodontol. 1992 Feb;63(2):131–136. doi: 10.1902/jop.1992.63.2.131. [DOI] [PubMed] [Google Scholar]
  21. Riviere G. R., Weisz K. S., Adams D. F., Thomas D. D. Pathogen-related oral spirochetes from dental plaque are invasive. Infect Immun. 1991 Oct;59(10):3377–3380. doi: 10.1128/iai.59.10.3377-3380.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rosey E. L., Kennedy M. J., Petrella D. K., Ulrich R. G., Yancey R. J., Jr Inactivation of Serpulina hyodysenteriae flaA1 and flaB1 periplasmic flagellar genes by electroporation-mediated allelic exchange. J Bacteriol. 1995 Oct;177(20):5959–5970. doi: 10.1128/jb.177.20.5959-5970.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Salomón R. A., Farías R. N. Influence of iron on microcin 25 production. FEMS Microbiol Lett. 1994 Sep 1;121(3):275–279. doi: 10.1016/0378-1097(94)90303-4. [DOI] [PubMed] [Google Scholar]
  24. Salomón R. A., Farías R. N. Microcin 25, a novel antimicrobial peptide produced by Escherichia coli. J Bacteriol. 1992 Nov;174(22):7428–7435. doi: 10.1128/jb.174.22.7428-7435.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Salomón R. A., Farías R. N. The FhuA protein is involved in microcin 25 uptake. J Bacteriol. 1993 Dec;175(23):7741–7742. doi: 10.1128/jb.175.23.7741-7742.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Salomón R. A., Farías R. N. The peptide antibiotic microcin 25 is imported through the TonB pathway and the SbmA protein. J Bacteriol. 1995 Jun;177(11):3323–3325. doi: 10.1128/jb.177.11.3323-3325.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. San Millan J. L., Hernandez-Chico C., Pereda P., Moreno F. Cloning and mapping of the genetic determinants for microcin B17 production and immunity. J Bacteriol. 1985 Jul;163(1):275–281. doi: 10.1128/jb.163.1.275-281.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. San Millán J. L., Kolter R., Moreno F. Plasmid genes required for microcin B17 production. J Bacteriol. 1985 Sep;163(3):1016–1020. doi: 10.1128/jb.163.3.1016-1020.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Simonson L. G., Goodman C. H., Bial J. J., Morton H. E. Quantitative relationship of Treponema denticola to severity of periodontal disease. Infect Immun. 1988 Apr;56(4):726–728. doi: 10.1128/iai.56.4.726-728.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Uitto V. J., Grenier D., Pan Y. M., McBride B., Cawston T. The collagenolytic activity of Treponema denticola. Matrix Suppl. 1992;1:141–142. [PubMed] [Google Scholar]
  31. Weinberg A., Holt S. C. Interaction of Treponema denticola TD-4, GM-1, and MS25 with human gingival fibroblasts. Infect Immun. 1990 Jun;58(6):1720–1729. doi: 10.1128/iai.58.6.1720-1729.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Wilson D. R., Beveridge T. J. Bacterial flagellar filaments and their component flagellins. Can J Microbiol. 1993 May;39(5):451–472. doi: 10.1139/m93-066. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES