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. Author manuscript; available in PMC: 2007 Jan 25.
Published in final edited form as: Virology. 2006 May 6;351(2):291–302. doi: 10.1016/j.virol.2006.03.042

Fig. 7.

Fig. 7

DNA packaging efficiency and cytopathic effect of core-labeled viruses. (A) 911 cells were infected over the course of 4 days with control Ad-wt-E3-EGFP (--○--), Ad-wt-E3-V-EGFP (–△–), and Ad-wt-E3-preVII-EGFP (–□–). Each day, half of the cells collected were processed for total viral DNA while the other half were processed for encapsidated viral DNA. Both viral DNA pools were quantitated by Taqman real-time quantitative PCR using E4-specific primers (n = 3). The third panel shows the percent of encapsidated viral DNA for each respective virus (encapsidated divided by total and then multiplied by 100%). (B) The cytopathic effect of the same three viruses was analyzed in 911 cells over the course of 10 days using the indicated multiplicities of infection. Every 2 days, the cell viability was quantitated by MTS assay. Cell viability is expressed as percentage relative to noninfected cells (n = 5). Ad-wt-E3-EGFP (--○--), Ad-wt-E3-V-EGFP (–△–), and Ad-wt-E3-preVII-EGFP (–□–).